Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice (Oryza sativa L.)
Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice (Oryza sativa L.)
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DOI：
10.1007/s00299-005-0077-2
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发表时间：
2006-05-01
影响因子：
6.2
通讯作者：
Watanabe, M
中科院分区：
文献类型：
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作者：
Park, JI;Hakozaki, H;Watanabe, M
在我们之前的cDNA微阵列分析中，我们在水稻中鉴定了53个成熟花药特异性基因，其功能未知。我们从特定氨基酸基序的角度重新分析了这些基因。在53个基因中，Os 26、Os32和Os 169（分别命名为OsSCP 1、OsSCP 2和OsSCP 3）3个基因编码富含半胱氨酸的基序（Cys-X-3-Cys-X-13-Cys-X-3-Cys），表明它们是新的富含半胱氨酸的小蛋白。通过对启动子区特异性元件的研究，发现了几个花粉特异性元件，为了确定这三个启动子在花粉中是否有功能，将启动子区与β-葡萄糖醛酸酶基因融合的基因构建体转化烟草。组织化学分析表明，这些启动子在成熟的花粉粒和花粉管中有活性。此外，OsSCP 1和OsSCP 3在水稻基因组中串联形成一个多基因家族。结果表明，OsSCPs可能在花粉发育和花粉管生长过程中发挥重要作用。
In our previous cDNA microarray analysis, we identified 53 mature anther-specific genes, whose function was unknown, in rice. We reanalyzed these genes from the viewpoint of the specific amino acid motif. Out of 53 genes, three genes, Os-26, Os-32, and Os-169 (renamed as OsSCP1, OsSCP2, and OsSCP3), encoded cysteine-rich motif (Cys-X-3-Cys-X-13-Cys-X-3-Cys), indicating that they were novel small cysteine-rich proteins. From the search of specific elements in promoter regions, several pollen-specific elements were found. In order to determine whether three promoters were functional in pollen or not, the gene constructs with promoter regions fused to the beta-glucuronidase gene were transformed into tobacco. Histochemical analysis showed that these promoters were active in the mature pollen grains and pollen tubes. Furthermore, OsSCP1 and OsSCP3 formed a multigene family tandemly in the rice genome. From the results, OsSCPs might have important roles in mature pollen development and pollen tube growth.