Dynamic localization of a cytoplasmic signal transduction response regulator controls morphogenesis during the Caulobacter cell cycle
Dynamic localization of a cytoplasmic signal transduction response regulator controls morphogenesis during the Caulobacter cell cycle
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DOI：
10.1073/pnas.051609998
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发表时间：
2001-03-27
影响因子：
11.1
通讯作者：
Shapiro, L
中科院分区：
文献类型：
--
作者：
Jacobs, C;Hung, D;Shapiro, L
我们提出的证据表明，将形态发生与细胞周期进展结合在一起的细菌信号转导级联受到其成分的动态定位的调节。以前的研究已经发现了两种组氨酸激酶。DiVJ和Plec，以及响应调节因子DivK，在二相性细菌新月弯杆菌的形态发生调控中的作用。在这里，我们发现细胞质反应调节因子D[VK]表现出动态的、周期性的定位，最终导致DivK在两种细胞类型中的不对称分布，这两种细胞是Caulbacter细胞周期的特征；DivK分散在后代沼泽细胞的细胞质中，并定位于柄细胞的极点。膜结合的DivJ和Plec组氨酸激酶不对称地定位于分裂前细胞的两极，控制着DivK的时空定位。DivJ介导DivK靶向极点，而Plec则在与DivJ和Plec在Tate前分裂细胞的活动和位置一致的时间和地点控制其从一个极点的释放。因此，信号转导级联的多个组成部分的亚细胞位置的动态变化可能构成一种新的原核生物调控模式，以产生和维持细胞不对称性。
We present evidence that a bacterial signal transduction cascade that couples morphogenesis with cell cycle progression is regulated by dynamic localization of its components. Previous studies have implicated two histidine kinases. DiVJ and PleC, and the response regulator, DivK, in the regulation of morphogenesis in the dimorphic bacterium Caulobacter crescentus. Here, we show that the cytoplasmic response regulator, D[VK, exhibits a dynamic, cyclical localization that culminates in asymmetric distribution of DivK within the two cell types that are characteristic of the Caulobacter cell cycle; DivK is dispersed throughout the cytoplasm of the progeny swarmer cell and is localized to the pole of the stalked cell. The membrane-bound DivJ and PleC histidine kinases, which are asymmetrically localized at the opposite poles of the predivisional cell, control the temporal and spatial localization of DivK. DivJ mediates DivK targeting to the poles whereas PleC controls its release from one of the poles at times and places that are consistent with the activities and location of DivJ and PleC in the tate predivisional cell. Thus, dynamic changes in subcellular location of multiple components of a signal transduction cascade may constitute a novel mode of prokaryotic regulation to generate and maintain cellular asymmetry.