Astroglial connexin 43 sustains glutamatergic synaptic efficacy
Astroglial connexin 43 sustains glutamatergic synaptic efficacy
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DOI：
10.1098/rstb.2013.0596
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发表时间：
2014-10-01
影响因子：
6.3
通讯作者：
Rouach, Nathalie
中科院分区：
文献类型：
--
作者：
Chever, Oana;Pannasch, Ulrike;Rouach, Nathalie
星形胶质细胞与神经元的动态相互作用在神经传递中起着积极的作用。差距连接（GJ）亚基连接蛋白43和30在星形胶质细胞中强烈表达，并且最近被证明调节突触活性和可塑性。然而，连接蛋白43在原位星形胶质细胞的形态和电生理特性以及突触传递中的具体作用仍然未知。在这里，我们表明，连接蛋白43，星形胶质细胞GJ耦合的一个主要决定因素，调节星形胶质细胞的细胞体积，但没有影响星形胶质细胞的被动膜性能。此外，我们证明，连接蛋白43调节海马CA1区锥体细胞的突触活性。这种调节涉及突触释放的谷氨酸的变化，而神经元兴奋性或突触后功能没有改变。这些结果揭示连接蛋白43通过支持突触功效在神经胶质相互作用中作为关键参与者。
Astrocytes dynamic interactions with neurons play an active role in neurotransmission. The gap junction (GJ) subunits connexins 43 and 30 are strongly expressed in astrocytes and have recently been shown to regulate synaptic activity and plasticity. However, the specific role of connexin 43 in the morphological and electrophysiological properties of astrocytes in situ as well as in synaptic transmission remains unknown. Here, we show that connexin 43, a major determinant of astroglial GJ coupling, regulates astrocyte cell volume, but has no impact on astroglial passive membrane properties. Furthermore, we demonstrate that connexin 43 modulates glutamatergic synaptic activity of hippocampal CA1 pyramidal cells. This regulation involves changes in synaptically released glutamate, with no alteration in neuronal excitability or postsynaptic function. These results reveal connexin 43 as a critical player in neuroglial interactions by supporting synaptic efficacy.