Isolation and potential immunological characterization of TPSGLVY, a novel bursal septpeptide isolated from the bursa of Fabricius
Isolation and potential immunological characterization of TPSGLVY, a novel bursal septpeptide isolated from the bursa of Fabricius
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DOI：
10.1016/j.peptides.2010.05.007
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发表时间：
2010-08-01
期刊：
影响因子：
3
通讯作者：
Chen, Puyan
中科院分区：
文献类型：
--
作者：
Feng, Xiuli;Su, Xiaodong;Chen, Puyan
法氏囊是鸟类特有的中枢免疫器官，其提取物具有刺激B细胞分化和增强抗体产生的免疫活性。然而，除了法氏囊素以外，从法氏囊中提取的活性多肽还鲜有报道。本文鉴定了一种新的法氏囊多肽(BSP-II)，其氨基酸序列为TPSGLVY，与鸡MGC53864蛋白相似。我们从抗体滴度(IgG1和IgG2α)、干扰素-γ和白介素4细胞因子水平、脾淋巴细胞增殖反应和T淋巴细胞亚群组成等方面研究了BSP-II对免疫应答的影响。值得注意的是，BSP-II以剂量依赖的方式增强Th1和Th2型免疫反应。BSP-II在两个不同的血清浓度(20%和5%)下对杂交瘤SP2/0细胞有特异性的促增殖作用，但与BSP-II的剂量无关。在5%和20%的血清中，随着BSP-II浓度的增加，杂交瘤SP2/0细胞的抗体分泌水平升高。BSP-II对肿瘤细胞(Hela和SP2/0)的存活率也有影响。这些结果表明，BSP-II能够显著诱导多种免疫反应，并参与不同肿瘤细胞系的细胞活性。结果提示，BSP-II可能是法氏囊中一种新的具有免疫调节活性的生物活性因子。皇冠版权所有(C)2010由爱思唯尔公司出版。保留所有权利。
The bursa of Fabricius is central immune organ unique to birds, and the extract is immunocompetent in stimulating B cell differentiation and enhancing antibody production. However, except for bursin, the active peptides from the bursa of Fabricius are little reported. In the paper, a novel bursal septpeptide (BSP-II) with the amino acids sequence of TPSGLVY was identified and similar to the MGC53864 protein of Gallus gallus. We investigated the effects of BSP-II on the immune response in terms of the antibodies titers (IgG1 and IgG2 alpha), the levels of interferon-gamma and interleukin-4 cytokines, spleen cell lymphocyte proliferation, and the T-lymphocyte subtype composition. It was noteworthy that BSP-II potentiates the Th1 and Th2-type immune responses in dose-dependent manner. BSP-II had specific enhancing effects on the hybridoma SP2/0 cell proliferation at two different serum concentrations (20% and 5%), but had no connection with the dose of BSP-II. The antibody secreting level of hybridoma SP2/0 cells rose in 5% and 20% serum when the concentrations of BSP-II increased. Also, BSP-II had effect on the viabilities of tumor cells (Hela and SP2/0). All the results indicated that BSP-II was able to significantly induce various immune responses and involved in the cell viability of different tumor cell lines. Our observations implied that BSP-II might be a novel biological active factor from the bursa of Fabricius with immunomodulatory activities. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.