Isolation and characterization of a phospholipase A2 inhibitor from the blood plasma of the Thailand cobra Naja naja kaouthia.
Isolation and characterization of a phospholipase A2 inhibitor from the blood plasma of the Thailand cobra Naja naja kaouthia.
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从泰国眼镜蛇 Naja naja kaouthia 血浆中分离和表征磷脂酶 A2 抑制剂。
DOI：
10.1006/bbrc.1994.1519
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发表时间：
1994
影响因子：
3.1
通讯作者：
K. Hayashi
中科院分区：
文献类型：
--
作者：
N. Ohkura;S. Inoue;K. Ikeda;K. Hayashi
通过在 Sephadex G-200、DEAE Affi-Gel Blue 和 Protein-Pak G-Butyl 柱上进行连续层析，从泰国眼镜蛇 Naja naja kaouthia 的血浆中纯化磷脂酶 A2 (PLA2) 抑制蛋白。纯化的抑制剂是表观分子量约为90 kDa的糖蛋白，含有摩尔比为1:2的25-和31-kDa亚基。 31-kDa 亚基含有糖苷链，经 N-糖苷酶 F 处理，分子量降至 28 kDa。这两个亚基的氨基酸组成的特点是半胱氨酸残基含量高。该抑制剂抑制 II 组和 I 组 PLA2。由于其基本特性与已报道的两种响尾蛇抑制剂不同，因此眼镜蛇抑制剂可能是一种新型的 PLA2 抑制剂。
A phospholipase A2 (PLA2) inhibitory protein was purified from the blood plasma of the Thailand cobra Naja naja kaouthia by sequential chromatography on Sephadex G-200, DEAE Affi-Gel Blue, and Protein-Pak G-Butyl columns. The purified inhibitor was a glycoprotein with an apparent molecular mass of about 90 kDa and contained 25- and 31-kDa subunits with a molar ratio of 1:2. The 31-kDa subunit contained a glycosidic chain and the molecular mass was reduced to 28 kDa by N-glycosidase F treatment. The amino acid compositions of the two subunits were characterized by their high content of cysteine residues. The inhibitor inhibited group II as well as group I PLA2's. Since the fundamental properties were different from those of the two Crotalidae inhibitors already reported, the cobra inhibitor might be a new type of PLA2 inhibitor.