Cloning and localization of two multigene receptor families in goldfish olfactory epithelium
Cloning and localization of two multigene receptor families in goldfish olfactory epithelium
复制标题
DOI：
10.1073/pnas.95.20.11987
复制
发表时间：
1998-09-29
影响因子：
11.1
通讯作者：
Stryer, L
中科院分区：
文献类型：
--
作者：
Cao, YX;Oh, BC;Stryer, L
金鱼的繁殖是由排卵期的雌性释放并被雄性检测到的信息素协调的。两个高度有效的信息素，二羟孕酮和前列腺素，以前已被确定，其对金鱼行为的影响已被深入研究。我们已经克隆了金鱼嗅上皮cDNA属于两个多基因G蛋白偶联受体家族作为一个步骤，阐明信息素识别的分子基础。一个基因家族（GFA）由在其他鱼类和哺乳动物的嗅上皮中发现的推定气味受体（约320个残基）的同源物组成。另一个家族（GFB）由哺乳动物犁鼻器（VNO）和河豚鼻子中发现的推定信息素受体的同系物组成。GFB受体（约840个残基）类似于VNO受体的V2 R家族，其具有大的细胞外N末端结构域，并且是钙敏感和代谢型谷氨酸受体的同源物。原位杂交结果显示，这两个家族的金鱼受体在嗅上皮中的表达存在差异。GFB mRNA在相当紧凑的细胞中是丰富的，其核靠近顶面。相比之下，GFA mRNA被发现在细长的细胞中，其核位于更深的上皮中。我们的研究结果支持这一假设，即单独的嗅觉器官和VNO的陆生脊椎动物出现在进化的不同类别的神经元，差异定位在嗅上皮的前体水生脊椎动物的隔离。
Goldfish reproduction is coordinated by pheromones that are released by ovulating females and detected by males. Two highly potent pheromones, a dihydroxyprogesterone and a prostaglandin, previously have been identified, and their effects on goldfish behavior have been studied in depth. We have cloned goldfish olfactory epithelium cDNAs belonging to two multigene G-protein coupled receptor families as a step toward elucidating the molecular basis of pheromone recognition. One gene family (GFA) consists of homologs of putative odorant receptors (approximate to 320 residues) found in the olfactory epithelium of other fish and mammals. The other family (GFB) consists of homologs of putative pheromone receptors found in the vomeronasal organ (VNO) of mammals and also in the nose of pufferfish. GFB receptors (approximate to 840 residues) are akin to the V2R family of VNO receptors, which possess a large extracellular N-terminal domain and are homologs of calcium-sensing and metabotropic glutamate receptors. In situ hybridization showed that the two families of goldfish receptors are differentially expressed in the olfactory epithelium. GFB mRNA is abundant in rather compact cells whose nuclei are near the apical surface. In contrast, GFA mRNA is found in elongated cells whose nuclei are positioned deeper in the epithelium. Our findings support the hypothesis that the separate olfactory organ and VNO of terrestrial vertebrates arose in evolution by the segregation of distinct classes of neurons that were differentially positioned in the olfactory epithelium of a precursor aquatic vertebrate.