First visualization of dopaminergic neurons with a monoclonal antibody to dopamine: a light and electron microscopic study.
First visualization of dopaminergic neurons with a monoclonal antibody to dopamine: a light and electron microscopic study.
复制标题
首次用多巴胺单克隆抗体可视化多巴胺能神经元：光和电子显微镜研究。
DOI：
10.1177/35.11.3309046
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
A. Calas
中科院分区：
文献类型：
--
作者：
C. Decavel;L. Lescaudron;N. Mons;A. Calas
最近合成的单克隆抗体对多巴胺（DA）进行了测试，在大鼠和小鼠脑切片进一步处理后，PAP免疫细胞化学在光镜和电镜水平。DA免疫反应阳性细胞体的分布在黑质（SN），腹侧被盖区（VTA），中缝核。DA免疫反应纤维进行了研究，在两个DA投射系统，纹状体和隔。在黑质和腹侧被盖区发现许多多巴胺能细胞体。SN网状部可见散在分布的DA能神经元。中缝背核和中缝线核的免疫反应阳性神经元稀疏，DA纤维丛密集。免疫反应纤维在整个纹状体中观察到，更密集的腹侧部分。在隔，免疫阴性神经元概述了薄DA纤维突触接触其胞体或树突。根据我们的观察，这种DA单克隆抗体似乎是一个选择性和敏感的工具，研究多巴胺能神经元回路在组织学和超微结构水平。
A monoclonal antibody recently synthesized against dopamine (DA) was tested in rat and mouse brain sections after further treatment by PAP immunocytochemistry at the light and electron microscopic levels. Distribution of DA-immunoreactive cell bodies was examined in the substantia nigra (sn), the ventral tegmental area (vta), and the raphe nuclei. DA-immunoreactive fibers were investigated in two DA projection systems, the striatum and the septum. Many dopaminergic cell bodies were found in the sn and the vta. Some scattered DA neurons were encountered in the pars reticulata of the sn. The dorsal raphe and linearis raphe nuclei displayed sparse immunoreactive neurons and a dense plexus of DA fibers. Immunoreactive fibers were observed in the entire striatum, more dense in the ventral part. In the septum, immunonegative neurons were outlined by thin DA fibers in synaptic contact with their somata or dendrites. According to our observations, this DA monoclonal antibody seems to be a selective and sensitive tool for studying the dopaminergic neuronal circuitry at both histological and ultrastructural level.
影响因子：
1.3
作者：
Riema Abdunnur
通讯作者：
Riema Abdunnur