A chemically defined carrier for the delivery of human mesenchymal stem/stromal cells to skin wounds.
A chemically defined carrier for the delivery of human mesenchymal stem/stromal cells to skin wounds.
复制标题
DOI：
10.1089/ten.tec.2011.0037
复制
发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
N. Walker;A. R. Mistry;L. Smith;P. Eves;G. Tsaknakis;S. Forster;S. Watt;S. MacNeil
中科院分区：
文献类型：
--
作者：
N. Walker;A. R. Mistry;L. Smith;P. Eves;G. Tsaknakis;S. Forster;S. Watt;S. MacNeil
皮肤具有非凡的再生能力，但与年龄和糖尿病相关的血管问题导致成千上万的英国患者出现慢性不愈合伤口。需要新的治疗方法来治疗这些耐药性伤口。供体间充质干细胞/基质细胞（MSC）已被证明可以通过加速上皮再形成来帮助皮肤伤口愈合。这项工作的目的是设计一种低风险且方便的递送方法，将这些细胞转移到伤口床。通过等离子体聚合，用丙烯酸对医用级有机硅的表面进行功能化。细胞很好地附着在这些载体上，并且在载体上培养长达3天并没有显着影响它们的表型或支持血管小管形成的能力。然后使用这些载体将间充质干细胞转移到人类真皮上。使用 MTT 测定来评估活细胞数量并增强绿色荧光蛋白标记的 MSC 来证实细胞转移，以证明细胞在转移后附着在真皮上。我们的结论是，这种合成载体膜是一种有前途的治疗性 MSC 递送方法，并为未来研究评估其对修复困难皮肤伤口的影响开辟了道路。
Skin has a remarkable capacity for regeneration, but age- and diabetes-related vascular problems lead to chronic non-healing wounds for many thousands of U.K. patients. There is a need for new therapeutic approaches to treat these resistant wounds. Donor mesenchymal stem/stromal cells (MSCs) have been shown to assist cutaneous wound healing by accelerating re-epithelialization. The aim of this work was to devise a low risk and convenient delivery method for transferring these cells to wound beds. Plasma polymerization was used to functionalize the surface of medical-grade silicone with acrylic acid. Cells attached well to these carriers, and culture for up to 3 days on the carriers did not significantly affect their phenotype or ability to support vascular tubule formation. These carriers were then used to transfer MSCs onto human dermis. Cell transfer was confirmed using an MTT assay to assess viable cell numbers and enhanced green fluorescent protein-labeled MSCs to demonstrate that the cells post-transfer attached to the dermis. We conclude that this synthetic carrier membrane is a promising approach for delivery of therapeutic MSCs and opens the way for future studies to evaluate its impact on repairing difficult skin wounds.