Resveratrol enhances intestinal barrier function by ameliorating barrier disruption in Caco-2 cell monolayers
Resveratrol enhances intestinal barrier function by ameliorating barrier disruption in Caco-2 cell monolayers
复制标题
DOI：
10.1016/j.jff.2018.10.009
复制
发表时间：
2018-12-01
影响因子：
5.6
通讯作者：
Suzuki, Takuya
中科院分区：
文献类型：
--
作者：
Mayangsari, Yunika;Suzuki, Takuya
已有研究表明，某些多酚类物质参与了屏障调节。在这里，我们研究了白藜芦醇对Caco-2细胞单层的影响，重点是对紧密连接(TJ)结构的调节。观察白藜芦醇对过氧化氢和白介素6诱导的细胞屏障损伤的影响。跨上皮电阻测量显示，白藜芦醇增强了TJ屏障的完整性。免疫印迹和免疫荧光分析表明，白藜芦醇上调TJ蛋白的表达及其在细胞骨架部分的分布。此外，白藜芦醇通过抑制封闭蛋白的酪氨酸磷酸化，改善了氧化应激诱导的屏障损伤的Caco-2细胞模型中过氧化氢诱导的上皮屏障破坏。研究发现，白藜芦醇通过下调Claudin-2的表达，减轻了IL-6诱导的炎症细胞模型的高通透性。结果表明，白藜芦醇增强TJ屏障的完整性，并介导TJ屏障对氧化应激和促炎细胞因子诱导的Caco-2细胞损伤的保护作用。
It has been shown that some polyphenols participate in barrier regulation. Here, we investigated the effects of resveratrol on Caco-2 cell monolayers, with a focus on the modulation of tight junction (TJ) structure. The effects of resveratrol on barrier impairment in the cells following treatment with H2O2 and interleukin (IL)-6 were investigated. Measurement of transepithelial electrical resistance showed that resveratrol enhances TJ barrier integrity. Immunoblot and immunofluorescence analyses revealed that resveratrol upregulates the expression of TJ proteins and their distribution in the cytoskeletal fraction. Furthermore, resveratrol ameliorated H2O2-induced epithelial barrier disruption in the Caco-2 cell model of oxidative-stress-induced barrier impairment by inhibiting tyrosine phosphorylation of occludin. It was found that resveratrol mitigated IL-6-induced hyperpermeability in the inflamed cell model by downregulating claudin-2 expression. The results show that resveratrol enhances TJ barrier integrity and mediates TJ barrier protection against damage induced by oxidative stress and pro-inflammatory cytokines in Caco-2 cells.