Far-field fluorescence nanoscopy of diamond color centers by ground state depletion
Far-field fluorescence nanoscopy of diamond color centers by ground state depletion
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DOI：
10.1209/0295-5075/86/14001
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发表时间：
2009-04-01
期刊：
影响因子：
1.8
通讯作者：
Hell, S. W.
中科院分区：
文献类型：
--
作者：
Rittweger, E.;Wildanger, D.;Hell, S. W.
我们报告两种模式的基于透镜的荧光显微镜与衍射无限的分辨率依赖于耗尽的荧光团基态。第一个版本利用具有深强度最小值的光束，例如甜甜圈，用于强烈的激发，然后进行数学去卷积，而在第二个版本中，添加了规则聚焦的光束，用于直接生成图像。与理论一致，亚衍射分辨率与耗尽基态的强度的平方根成比例。应用于钻石色心的成像，我们的测量证据的分辨率下降到约7.6 nm，对应于1/70的光波长。我们的研究强调了在远场光学显微镜中利用（分子）态克服衍射势垒的关键作用。版权所有（C）EPLA，2009
We report on two modalities of lens-based fluorescence microscopy with diffraction-unlimited resolution relying on the depletion of the fluorophore ground state. The first version utilizes a beam with a deep intensity minimum, such as a doughnut, for intense excitation followed by mathematical deconvolution, whereas in the second version, a regularly focused beam is added for generating the image directly. In agreement with theory, the subdiffraction resolution scales with the square root of the intensity depleting the ground state. Applied to the imaging of color centers in diamond our measurements evidence a resolving power down to approximate to 7.6 nm, corresponding to 1/70 of the wavelength of light employed. Our study underscores the key role of exploiting (molecular) states for overcoming the diffraction barrier in far-field optical microscopy. Copyright (C) EPLA, 2009