Synthetic Octacalcium Phosphate Augments Bone Regeneration Correlated with Its Content in Collagen Scaffold
Synthetic Octacalcium Phosphate Augments Bone Regeneration Correlated with Its Content in Collagen Scaffold
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DOI：
10.1089/ten.tea.2008.0141
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发表时间：
2009-01-01
影响因子：
4.1
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
--
作者：
Kawai, Tadashi;Anada, Takahisa;Suzuki, Osamu
以前的研究表明，合成的磷酸八钙（OCP）促进体外成骨细胞分化的OCP剂量依赖性的方式和OCP和胶原蛋白（OCP/胶原蛋白）的复合物增强临界大小的大鼠颅骨缺损比OCP单独。本研究旨在研究OCP/胶原蛋白的骨再生性能是否以OCP剂量依赖性方式增强，从而建立这种复合材料作为骨替代材料的合适组合物。通过将OCP颗粒与去端肽胶原共混来制备具有23：77至83：17的宽范围混合比的OCP/胶原，包括先前检查的组合物（77：23），并将其模制成盘作为植入物。在大鼠颅盖骨中制造一个临界大小的缺损，将每个圆盘植入缺损处4或12周，然后进行放射学、组织学和组织形态学检查。将小鼠骨髓源性基质ST-2细胞培养在预先包被有OCP/胶原蛋白或单独具有不同OCP含量的OCP的培养皿中，以确定细胞粘附和增殖的能力长达14天。组织学和放射学检查显示，观察到与胶原基质内的OCP颗粒相关的新形成的骨。组织形态学分析证实，增加胶原基质中OCP的量导致骨再生的逐步增强，并且比例83：17在12周时产生约64%的缺损的最大修复水平。OCP/胶原促进ST-2细胞的增殖和附着比OCP单独使用，无论OCP含量。温育后涂层的傅立叶变换红外光谱分析表明，无论胶原蛋白的存在，OCP倾向于转化为磷灰石。本研究表明，OCP/胶原的骨传导特性可以以OCP剂量依赖性方式显示。结果表明，胶原促进宿主成骨细胞在OCP/胶原复合植入物上的增殖和附着。
Previous studies have shown that synthetic octacalcium phosphate (OCP) facilitates in vitro osteoblastic cell differentiation in an OCP dose-dependent manner and that a complex of OCP and collagen (OCP/collagen) enhances critical-sized rat calvaria defects more than OCP alone. The present study was designed to investigate whether the bone regenerative properties of OCP/collagen are augmented in an OCP dose-dependent manner, thereby establishing a suitable composition of this composite as a bone substitute material. OCP/collagens with a wide range of mixing ratios from 23: 77 to 83: 17, including the previously examined composition (77: 23), were prepared by blending granules of OCP with atelocollagen and molded into a disk as an implant. A critical-sized defect was made in rat calvaria, and each disk was implanted into the defect for 4 or 12 weeks and then examined radiographically, histologically, and histomorphometrically. Mouse bone marrow-derived stromal ST-2 cells were cultured in dishes pre-coated with OCP/collagen or OCP alone with different OCP contents to determine the capacity of cell attachment and proliferation up to 14 days. Histological and radiographic examinations showed that newly formed bone was observed in relation to OCP granules within the collagen matrix. Histomorphometric analysis confirmed that increasing the amount of OCP in collagen matrices resulted in progressive enhancement of bone regeneration and that the ratio 83: 17 generated the maximum repair level of approximately 64% of the defect at 12 weeks. OCP/collagen promoted the proliferation and attachment of ST-2 cells more than OCP alone regardless of OCP content. Fourier transform infrared spectroscopy analysis of the coatings after the incubation indicated that OCP tended to convert to apatite regardless of the presence of collagen. The present study demonstrated that the osteoconductive characteristics of OCP/collagen can be displayed in an OCP dose-dependent manner. The results suggest that collagen promotes the proliferation and attachment of host osteoblastic cells on OCP/collagen composite implants.