The differentiation of germ cells and gonads during development of carp (Cyprinus carpio L.). A study with anti-carp sperm monoclonal antibodies.
The differentiation of germ cells and gonads during development of carp (Cyprinus carpio L.). A study with anti-carp sperm monoclonal antibodies.
复制标题
DOI：
--
复制
发表时间：
1985-12
期刊：
影响因子：
--
通讯作者：
H. Parmentier;L. Timmermans
中科院分区：
文献类型：
--
作者：
H. Parmentier;L. Timmermans
研究了鲤鱼（Cyprinus carpio L.）仔、幼鱼性腺发育、生殖细胞分化及雌雄生殖细胞特异性膜抗原决定簇的出现。直到受精后25周。用4种抗鲤鱼精子单克隆抗体进行的间接免疫荧光研究表明，单克隆抗体WCS 29从受精后3天开始染色幼鱼原始生殖细胞的外膜。单克隆抗体WCS 3和17与受精后7周起的生殖细胞外膜反应，同时生殖细胞增殖的开始。从受精后18周开始，用单克隆抗体WCS 28生殖细胞膜被清楚地染色。在两种性别中观察到类似的反应，然而，雌性生殖细胞在较早的发育阶段与单克隆抗体WCS 28反应，而不是雄性生殖细胞。在发育中的睾丸中，单克隆抗体染色所有类型的生精细胞。在卵巢中，只有卵原细胞和早期前期卵母细胞与四种单克隆抗体呈阳性反应。结果表明，生殖系特异性抗原存在于原始生殖细胞及其雄性和雌性后代的外膜上，除老年产卵期外。据推测，这些膜抗原的出现和消失反映了生殖细胞在性腺发生过程中的分化步骤。
Gonadal development, germ cell differentiation and the appearance of membrane antigenic determinants, specific for male and female germ cells during gonadogenesis, was studied in larval and juvenile carp (Cyprinus carpio L.) until 25 weeks after fertilization. Indirect immunofluorescence studies with four monoclonal antibodies raised against carp spermatozoa revealed that monoclonal antibody WCS 29 stained the outer membranes of primordial germ cells in larvae from 3 days after fertilization. The monoclonal antibodies WCS 3 and 17 reacted with the outer membranes of germ cells from 7 weeks after fertilization onwards, simultaneously with the onset of germ cell proliferation. With monoclonal antibody WCS 28 germ cell membranes were clearly stained from 18 weeks after fertilization. Similar reactions were observed in both sexes, however, female germ cells reacted at an earlier developmental stage with the monoclonal antibody WCS 28 than male germ cells. In the developing testis the monoclonal antibodies stained all types of spermatogenic cells. In the ovary, however, only oogonia and early prophase oocytes showed a positive reaction with the four monoclonal antibodies. The results indicate that germline-specific antigens are present on the outer membranes of primordial germ cells and their male and female descendants, with the exception of elderly oögenic stages. It is assumed that the appearance and disappearance of these membrane antigens reflect differentiation steps of germ cells during gonadogenesis.