CELL-SHAPE AND ORGANIZATION OF THE CONTRACTILE APPARATUS IN CULTURED ADULT CARDIAC MYOCYTES
CELL-SHAPE AND ORGANIZATION OF THE CONTRACTILE APPARATUS IN CULTURED ADULT CARDIAC MYOCYTES
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DOI：
10.1016/0022-2828(91)90215-8
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发表时间：
1991-07-01
影响因子：
5
通讯作者：
DECKER, RS
中科院分区：
文献类型：
--
作者：
DECKER, ML;BEHNKEBARCLAY, M;DECKER, RS
成人心肌细胞的分离和培养已被证明是在细胞水平上探索心肌生物学的理想模型系统。然而，对该模型的主要批评是器官特异性特征（例如细胞形状和亚细胞结构组织）无法在体外长时间保留。将新鲜分离的肌细胞包裹在海藻酸钙基质中，可以保持培养的肌细胞的棒状三维 (3D) 形状。与在二维 (2D) 塑料表面上培养的心脏细胞相比，此类制剂在收缩装置、横管系统和肌浆网的组织方面更类似于体内的对应物。体视学测量显示，两种非搏动制剂中的肌原纤维体积密度 (VvMYF) 在两周内均有所下降，但与维持在层粘连蛋白包被的基质上的肌细胞相比，在藻酸盐基质中培养的细胞中，VvMYF 是保守的。目前的观察表明，在缺乏收缩功能的情况下，肌原纤维萎缩似乎是藻酸盐 (3D) 制剂中 VvMYF 下降的原因，而萎缩和亚细胞重塑可能介导在 2D 表面培养成体心脏细胞时发生的肌原纤维损失和重组。
The isolation and culture of adult cardiac myocytes has proved to be an ideal model system to explore myocardial biology at the cellular level. A major criticism of this model, however, has been that organ-specific characteristics such as cell shape and subcellular structural organization cannot be retainedin vitrofor prolonged periods of time. Encasing freshly isolated myocytes in a matrix of calcium alginate enables one to maintain the rod-like, three-dimensional (3D) shape of the cultured myocyte. Such preparations more closely resemble theirin vivocounterparts with respect to the organization of the contractile apparatus, the transverse tubular system and the sarcoplasmic reticulum than do heart cells cultured on a two-dimensional (2D) plastic surface. Stereologic measurements reveal that myofibrillar volume density (VvMYF) decreases in both non-beating preparations over a 2-week interval, butVvMYF is conserved in cells cultured in an alginate matrix when compared to those myocytes maintained on a laminin-coated substratum. The present observations suggest that in the absence of contractile function myofibrillar atrophy appears responsible for the decline inVvMYF in alginate (3D) preparations, whereas atrophy and subcellular remodelling probably mediate the myofibrillar loss and reorganization that develops when adult heart cells are cultured on a 2D surface.