Genome-wide association studies demonstrate the genes associated with perimysial thickness in ducks.
Genome-wide association studies demonstrate the genes associated with perimysial thickness in ducks.
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DOI：
10.1111/age.13297
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发表时间：
2023-01
期刊：
影响因子：
2.4
通讯作者：
Hehe Tang;Dapeng Liu;Huiling Zhang;W. Fan;Jian Hu;Yaxi Xu;Zhanbao Guo;Wei Huang;S. Hou;Zhengkui Zhou
中科院分区：
文献类型：
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作者：
Hehe Tang;Dapeng Liu;Huiling Zhang;W. Fan;Jian Hu;Yaxi Xu;Zhanbao Guo;Wei Huang;S. Hou;Zhengkui Zhou
肌围的厚度对肉的嫩度有重要影响。然而，肌膜周围厚度的遗传基础尚未确定。本研究的目的是利用全基因组关联研究的方法，探索影响绿头鸭×北京鸭F2分离群体肌膜厚度的数量性状基因座(QTL)。在27号和13号染色体上发现的两个QTL在全基因组水平上与肌周厚度性状显著相关。关联最强的是位于27号染色体上的QTL，该区域影响肌膜厚度，并包含一个有希望的候选基因MAGI3(膜相关鸟苷酸激酶，WW和PDZ结构域包含3)。关联分析表明，MAGI3基因的顶部SNP也与肌内脂肪含量性状相关，肌周厚度与肌内脂肪含量呈正相关。第二强关联是13号染色体上的QTL区域。SUCLG2(琥珀酸辅酶A连接酶GDP形成亚基β)位于SNP顶端，是另一个突出的候选基因。此外，转座酶可及染色质测序结果表明，一些与肌肉发育或能量代谢相关的关键转录因子(MYF5、MYOD1、KLF11)可能与MAGI3和SUCLG2的开放区域结合。通过分析候选基因不同基因型的表达情况，推测MAGI3的不同基因型可能对胸肌发育产生影响，进而影响肌束膜厚度。本研究定位了鸭胸肌肌周厚度性状的两个主效基因，这有助于确定肌肉发育的特征，并有助于家畜产肉量和品质的遗传改良。
The thickness of the perimysium has an essential effect on the tenderness of the meat. However, the genetic basis underlying perimysial thickness has not been determined. The objective of this study was to explore the quantitative trait loci (QTL) that influence perimysial thickness in an F2 segregating population generated by Mallard × Pekin duck using the genome-wide association study (GWAS) method. Two QTL identified in chromosomes 27 and 13 displayed significant associations with perimysial thickness traits at the genome-wide level. The strongest association was the QTL located in chromosome 27, and this region had an effect on perimysial thickness and contained a promising candidate gene MAGI3 (Membrane-associated guanylate kinase, WW and PDZ domain containing 3). Meanwhile, association analysis showed that the top SNP within the MAGI3 gene was also associated with intramuscular fat content traits, which showed that perimysial thickness was positively correlated with intramuscular fat content. The second strongest association was the QTL region of chromosome 13. SUCLG2 (Succinate-CoA ligase GDP-forming subunit beta) is proximal to the top SNP and stood out as another candidate gene. Furthermore, the Transposase-Accessible Chromatin using Sequencing result showed that some key transcription factors (MYF5, MYOD1, KLF11) related to muscle development or energy metabolism might bind to the open regions of MAGI3 and SUCLG2. By analyzing the expression of different genotypes of the candidate gene, we speculate that different genotypes of MAGI3 may have an effect on breast muscle development, and then affect the thickness of the perimysium. This study maps two major genes of the duck breast muscle perimysial thickness trait, which helps to characterize muscle development and contributes to the genetic improvement of meat yield and quality in livestock.