Echocardiographic assessment of cardiac function in conscious and anesthetized mice
Echocardiographic assessment of cardiac function in conscious and anesthetized mice
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DOI：
10.1152/ajpheart.1999.277.5.h1967
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发表时间：
1999-11-01
影响因子：
4.8
通讯作者：
Carretero, OA
中科院分区：
文献类型：
--
作者：
Yang, XP;Liu, YH;Carretero, OA
使用高频线性换能器（15 L 8），我们研究了1）与给予戊巴比妥钠（Pento）或氯胺酮和甲苯噻嗪混合物的小鼠相比，在非麻醉小鼠中进行超声心动图的可行性，以及2）超声心动图评价双肾、单夹高血压或心肌梗死（MI）小鼠左心室（LV）肥大、扩张和功能的可行性。清醒小鼠的心率（HR）为658 +/- 9次/min; Pento和氯胺酮+甲苯噻嗪分别将HR降低至377 +/- 11和293 +/- 19次/min，与缩短分数（SF）、射血分数（EF）和心输出量（CO）显著降低以及LV舒张末期（LVEDD）和收缩末期尺寸（LVESD）增加相关。两肾一夹高血压4周的小鼠左室质量增加（15.62 +/- 0.62对22.17 +/- 1.79 mg），而没有改变LV尺寸、SF、EF或CO。MI后4周研究的小鼠表现出明显的LV扩张和收缩功能障碍，表现为LVEDD和LVESD增加以及SF、EF、我们的研究结果清楚地表明了麻醉对基础心脏功能的不利影响，以及解释从麻醉小鼠获得的数据的困难。我们相信这是第一个研究，以证明使用超声心动图来评估心血管功能的非麻醉小鼠的可行性。
Using a high-frequency linear transducer (15L8), we studied 1) the feasibility of performing echocardiography in nonanesthetized mice compared with mice given pentobarbital sodium (Pento) or a mixture of ketamine and xylazine and 2) the feasibility of echocardiographic evaluation of left ventricular (LV) hypertrophy, dilatation, and function in mice with two-kidney; one-clip hypertension or myocardial infarction (MI). Heart rate (HR) in awake mice was 658 +/- 9 beats/min; Pento and ketamine plus xylazine reduced HR to 377 +/- 11 and 293 +/- 19 beats/min, respectively, associated with a significant decrease in shortening fraction (SF), ejection fraction (EF), and cardiac output (CO) and an increase in LV end-diastolic (LVEDD) and end-systolic dimensions (LVESD). Mice with 4 wk of two-kidney, one-clip hypertension had increased LV mass (15.62 +/- 0.62 vs. 22.17 +/- 1.79 mg) without altered LV dimensions, SF, EF, or CO. Mice studied 4 wk post-MI exhibited obvious LV dilatation and systolic dysfunction, as evidenced by increased LVEDD and LVESD and decreased SF, EF, and CO. Our findings clearly show the adverse impact of anesthesia on basal cardiac function and the difficulty in interpreting data obtained from anesthetized mice. We believe this is the first study to demonstrate the feasibility of using echocardiography to assess cardiovascular function in the nonanesthetized mouse.