AAC(1) - A NEW AMINOGLYCOSIDE-ACETYLATING ENZYME MODIFYING THE CL AMINOGROUP OF APRAMYCIN
AAC(1) - A NEW AMINOGLYCOSIDE-ACETYLATING ENZYME MODIFYING THE CL AMINOGROUP OF APRAMYCIN
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DOI：
10.1093/jac/20.6.803
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发表时间：
1987-12-01
影响因子：
5.2
通讯作者：
REEVES, DS
中科院分区：
文献类型：
--
作者：
LOVERING, AM;WHITE, LO;REEVES, DS
对具有不寻常耐药表型的大肠杆菌菌株产生的氨基糖苷乙酰化酶进行了表征。发现该酶可单乙酰化安普霉素、布替罗辛、青维霉素和巴龙霉素，并将核糖霉素和新霉素二乙酰化，得到反应产物，通过 HPLC 分析可将其与 AAC(2'')、AAC(3) 和 AAC(6'') 酶的反应产物区分开来。然而，未发现该酶乙酰化阿米卡星、福替米星、遗传霉素、庆大霉素、卡那霉素 A、奈替米星或妥布霉素。该酶对安普霉素作用的反应产物经过纯化并通过核磁共振研究鉴定为 1-N-乙酰基安普霉素。核霉素和新霉素 B 被该酶修饰的第二个位点尚未确定，但推测为 6''-氨基。建议将该酶命名为AAC(1)。
A aminoglycoside-acetylating enzyme produced by a strain of Escherichia coli with an unusual resistance phenotype was characterized. This enzyme was found to mono-acetylate apramycin, butirosin, lividomycin and paromomycin and diacetylate ribostamycin and neomycin to give reaction products which were distinguishable by HPLC analysis from those of AAC(2''), AAC(3) and AAC(6'') enzymes. The enzyme, however, was not found to acetylate amikacin, fortimicin, geneticin, gentamicin, kanamycin A, netilmicin or tobramycin. The reaction product from the action of this enzyme on apramycin was purified and identified by nuclear magnetic resonance studies as 1-N-acetyl apramycin. The second site at which ribostamycin and neomycin B were modified by this enzyme was not determined but is postulated as the 6''-amino group. It is proposed that this enzyme be named AAC(1).