The regulation of primary mesenchyme cell patterning.
The regulation of primary mesenchyme cell patterning.
复制标题
原代间充质细胞模式的调节。
DOI：
10.1016/0012-1606(90)90076-u
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发表时间：
1990
影响因子：
2.7
通讯作者：
Ettensohn,CA
中科院分区：
文献类型：
--
作者：
Ettensohn,CA
海胆胚胎的初级间充质细胞（PMC）经历了一系列引人注目的形态发生行为，包括迁移，定位在胚胎内的特定位点，以及幼虫骨骼的合成。为了获得关于这些过程是如何调节的信息，在具有实验改变的PMC数量的胚胎中分析PMC迁移和图案化。PMC移动后，用荧光染料罗丹明B异硫氰酸酯或PMC特异性单克隆抗体6a9标记细胞。这些方法表明，个别PMC有能力加入模式中的任何位置，并排除PMC形态发生涉及分选出离散的细胞亚群到预定位点的可能性。PMC模式中的所有站点都有能力接受比正常情况下更多的小区，并且PMC在该模式中似乎不会相互竞争首选站点。即使在具有2 - 3倍于正常PMC补体的胚胎中，所有这些细胞都参与骨针发生，并且所产生的骨骼在大小和构型上是正常的。两个特殊的网站沿着基膜（那些对应的PMC腹外侧集群的位置）促进骨针伸长，在这些网站的PMC的数量是独立的效果。这些观察强调了基膜，囊胚腔基质，胚胎上皮细胞在调节PMC形态发生的关键方面的作用。PMC保持高度灵活的能力，以应对图案线索的囊胚腔，因为迁移后的PMC将重复其图案的过程，如果显微注射到年轻的受体胚胎的囊胚腔。
The primary mesenchyme cells (PMCs) of the sea urchin embryo undergo a dramatic sequence of morphogenetic behaviors that includes migration, localization at specific sites within the embryo, and synthesis of the larval skeleton. To gain information about how these processes are regulated, PMC migration and patterning were analyzed in embryos with experimentally altered numbers of PMCs. PMC movements were followed by labeling the cells with a fluorescent dye, rhodamine B isothiocyanate, or with the PMC-specific monoclonal antibody 6a9. These methods show that individual PMCs have the capacity to join any position in the pattern, and rule out the possibility that PMC morphogenesis involves a sorting out of discrete subpopulations of cells to predetermined sites. All sites in the PMC pattern have the capacity to accept more cells than they normally do, and PMCs do not appear to compete with one another for preferred sites in the pattern. Even in embryos with 2–3 times the normal complement of PMCs, all these cells take part in spiculogenesis and the resultant skeleton is normal in size and configuration. Two special sites along the basal lamina (those corresponding to the positions of the PMC ventrolateral clusters) promote spicule elongation, an effect that is independent of the numbers of PMCs at these sites. These observations emphasize the role of the basal lamina, blastocoel matrix, and embryonic epithelium in regulating key aspects of PMC morphogenesis. The PMCs remain highly flexible in their ability to respond to patterning cues in the blastocoel, since postmigratory PMCs will repeat their patterning process if microinjected into the blastocoel of young recipient embryos.