A broad-host-range, generalized transducing phage (SN-T) acquires 16S rRNA genes from different genera of bacteria
A broad-host-range, generalized transducing phage (SN-T) acquires 16S rRNA genes from different genera of bacteria
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DOI：
10.1128/aem.71.12.8301-8304.2005
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发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Robinson, JB
中科院分区：
文献类型：
--
作者：
Beumer, A;Robinson, JB
基因组分析揭示了同一种细菌16S rRNA基因序列的异质性；然而，其原因仍不确定。广义转导噬菌体最近因其丰富的数量以及影响横向基因转移和容纳细菌16S rRNA基因序列的能力而获得认可。在这里，我们证明了广泛的宿主范围，广义的转导噬菌体获得16S rRNA基因和基因序列的能力。利用PCR和16S rRNA基因保守区域特异性引物，我们发现一般的转导噬菌体（D3112、UTI和SN-T），而不是特化的转导噬菌体（D3），获得了完整的细菌16S rRNA基因。此外，我们发现广泛的宿主范围，广义的转导噬菌体SN-T能够从两个不同的属获得16S rRNA基因：Sphaerotilus natans（最初分离SN-T的宿主）和铜绿假单胞菌。在序列感染中，根据限制性片段长度多态性分析，SN-T只携带最终宿主的16S rRNA基因序列。测定SN-T群体中16S rRNA基因序列的频率为1 × 10（-9）个转导物/PFU。我们的发现进一步暗示了16S rRNA基因在不同种类或属细菌之间水平转移的转导。
Genomic analysis has revealed heterogeneity among bacterial 16S rRNA gene sequences within a single species; yet the cause(s) remains uncertain. Generalized transducing bacteriophages have recently gained recognition for their abundance as well as their ability to affect lateral gene transfer and to harbor bacterial 16S rRNA gene sequences. Here, we demonstrate the ability of broad-host-range, generalized transducing phages to acquire 16S rRNA genes and gene sequences. Using PCR and primers specific to conserved regions of the 16S rRNA gene, we have found that generalized transducing phages (D3112, UTI, and SN-T), but not specialized transducing phages (D3), acquired entire bacterial 16S rRNA genes. Furthermore, we show that the broad-host-range, generalized transducing phage SN-T is capable of acquiring the 16S rRNA gene from two different genera: Sphaerotilus natans, the host from which SN-T was originally isolated, and Pseudomonas aeruginosa. In sequential infections, SN-T harbored only 16S rRNA gene sequences of the final host as determined by restriction fragment length polymorphism analysis. The frequency of 16S rRNA gene sequences in SN-T populations was determined to be 1 x 10(-9) transductants/PFU. Our findings further implicate transduction in the horizontal transfer of 16S rRNA genes between different species or genera of bacteria.