Multiple changes in gene expression in chronic human Achilles tendinopathy
Multiple changes in gene expression in chronic human Achilles tendinopathy
复制标题
DOI：
10.1016/s0945-053x(01)00128-7
复制
发表时间：
2001-06-01
期刊：
影响因子：
6.9
通讯作者：
Riley, G
中科院分区：
文献类型：
--
作者：
Ireland, D;Harrall, R;Riley, G
Atlas(TM) cDNA细胞相互作用阵列(CLONTECH)被用来检查四名跟腱疾病患者的退化组织，以鉴定在细胞-细胞和细胞-基质相互作用中重要的基因表达的变化。正常（死后）和退化组织样本之间的最大差异在于 MMP-3（基质溶解素）mRNA 水平，该水平在所有退化样本中均下调。对手术期间取自肌腱的成对“正常”和退化跟腱组织样本中提取的 RNA 进行定量 RT-PCR 分析，反映了阵列的结果。与“正常”样本相比，退化样本中 MMP-3 mRNA 的水平较低，而 I 型和 III 型胶原蛋白 mRNA 的水平显着较高。从相同肌腱样本中提取的蛋白质的免疫印迹显示，取自没有明显肌腱疾病的个体的四个正常组织样本中的三个的 MMP-3 蛋白水平比来自肌腱变性患者的“正常”或退化样本要高得多。我们认为MMP-3可能在肌腱细胞外基质降解和组织重塑的调节中发挥重要作用。 (C) 2001 Elsevier Science B.V./国际基质生物学学会。版权所有。
Atlas(TM) cDNA cell interaction arrays (CLONTECH) were used to examine degenerate tissue from four patients with Achilles tendon disorders, in order to identify changes in expression of genes important in cell-cell and cell-matrix interactions. The greatest difference between normal (post-mortem) and degenerate tissue samples was in the level of MMP-3 (stromelysin) mRNA, which was down-regulated in all the degenerate samples. Quantitative RT-PCR assay of RNA extracted from paired 'normal' and degenerate Achilies tendon tissue samples taken from tendons during surgery mirrored the results of the arrays. Levels of MMP-3 mRNA were lower, whereas levels of type-I and type-III collagen mRNAs were significantly higher, in the degenerate compared to the 'normal' samples. Immunoblotting of proteins extracted from the same tendon samples showed that three of four normal tissue samples taken from individuals without apparent tendon disorder had much higher levels of MMP-3 protein than 'normal' or degenerate samples from patients with tendinosis. We suggest that MMP-3 may play an important role in the regulation of tendon extracellular matrix degradation and tissue remodelling. (C) 2001 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.