Detection of bacterial DNA in atherornatous plaques by quantitative PCR
Detection of bacterial DNA in atherornatous plaques by quantitative PCR
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DOI：
10.1016/j.micinf.2005.09.004
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发表时间：
2006-03-01
影响因子：
5.8
通讯作者：
Lopatin, Dennis
中科院分区：
文献类型：
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作者：
Kozarov, Emil;Sweier, Domenica;Lopatin, Dennis
这是第一项分析动脉粥样硬化斑块中存在的10种细菌DNA的研究，包括牙周菌和肺炎衣原体。我们检查了从29个个体的动脉粥样硬化中提取的129个DNA样本，以确定是否存在来自10个不同物种的细菌16S rDNA序列：牙龈卟啉单胞菌，放线菌放线杆菌（A.a.），革兰氏阴性杆菌、革兰氏阴性杆菌、革兰氏阴性肺炎。所有测定均采用SYBR（（R））绿色实时定量聚合酶链反应（PCR）方法进行。在年轻患者中发现了约17%的拟杆菌属物种，但在老年患者中发现了约80%的拟杆菌属物种。几乎一半的样本含有A. a.和C.肺炎，虽然后者的比例很小。S.金黄色葡萄球菌和表皮样肉芽肿的发生率最低，分别为5%和10%。S.在大约20%的样本中发现了变形杆菌。计算每种细菌物种相对于原核DNA总量的比例。这些数据支持了我们以前的发现，牙周微生物和血管炎症之间的关联。我们的结论是，口腔感染因子的DNA在年轻人的动脉粥样硬化中很常见，尤其是老年人，C。肺炎的炎症可能是最小的。(c)2005年，Elsevier SAS。All rights reserved.
This is the first study to analyze atheromatous plaques for the presence of bacterial DNA from ten species, including periodontal species and Chlamydia pneumoniae. We examined 129 samples of DNA extracted from atheromas from 29 individuals for the presence of bacterial 16S rDNA sequences from ten different species: Porphyromonas gingivalis, Actinobacillus actinomycetenicomitans (A.a.), Tannerella forsythensis, Eikenella corrodens, Prevotella intermedia, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Treponema denticola and C. pneumoniae. All determinations were made using real-time quantitative polymerase chain reaction (PCR) methods employing SYBR((R))Green. Species from the Bacteroides family were found in about 17% of the young but similar to 80% in elderly patients. Almost half of the samples contained DNA from A. a. and C. pneumoniae, although the proportion of the latter was minimal. S. aureus and S. epidermidis were found with the lowest frequency, 5 and 10%, respectively. S. mutans was found in similar to 20% of the samples. The proportions of each bacterial species were calculated relative to the total amount of prokaryotic DNA. The data support our previous findings of an association between periodontal organisms and vascular inflammation. We conclude that DNA from oral infectious agents is commonly found in atheromas from young but especially from elderly subjects, and that the contribution of C. pneumoniae to the inflammation may be minimal. (c) 2005 Elsevier SAS. All rights reserved.