Apatite-coated poly(lactic-co-glycolic acid) microspheres as an injectable scaffold for bone tissue engineering
Apatite-coated poly(lactic-co-glycolic acid) microspheres as an injectable scaffold for bone tissue engineering
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DOI：
10.1002/jbm.a.31572
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发表时间：
2008-06-01
影响因子：
4.9
通讯作者：
Kim, Byung-Soo
中科院分区：
文献类型：
--
作者：
Kang, Sun-Woong;Yang, Hee Seok;Kim, Byung-Soo
生物可降解聚合物/陶瓷复合支架可以克服生物可降解聚合物或陶瓷在骨再生方面的局限性。可注射性骨再生支架材料由于其能够通过微创手术填充不规则形状的骨缺损和植入成骨细胞而引起了人们的极大兴趣。本研究的目的是确定磷灰石涂层聚乳酸-羟基乙酸共聚物（PLGA）微球是否可以用作可注射性骨再生支架材料。通过在模拟体液中孵育PLGA微球来制备磷灰石涂层PLGA微球。通过扫描电子显微镜、X射线衍射光谱、能量色散光谱和傅立叶变换红外光谱分析，发现包覆PLGA微球表面的磷灰石与天然骨中的磷灰石相似。将大鼠成骨细胞与磷灰石包被的PLGA微球混合，并立即注射到无胸腺小鼠的皮下部位。成骨细胞移植与普通PLGA微球作为对照。在6周时用苏木精和伊红染色、Masson三色染色和von Kossa染色对植入物进行组织学分析，结果显示磷灰石涂层PLGA微球组的骨再生比普通PLGA微球组好得多。磷灰石涂层PLGA微球组的新骨形成面积和种植体的钙含量明显高于普通PLGA微球组。本研究证明了使用磷灰石涂层PLGA微球作为体内骨组织工程的可注射支架的可行性。这种支架可用于通过骨科应用中的微创外科手术进行骨再生。(C)2007 Wiley Periodicals，Inc.
Biodegradable polymer/ceramic composite scaffold could overcome limitations of biodegradable polymers or ceramics for bone regeneration. Injectable scaffold has raised great interest for bone regeneration in vivo, since it allows one for easy filling of irregularly shaped bone defects and implantation of osteogenic cells through minimally invasive surgical procedures The purpose of this study was to determine whether apatite-coated poly(lactic-co-glycolic acid) (PLGA) microspheres could be used as an injectable scaffold to regenerate bone in vivo. Apatite-coated PLGA microspheres were fabricated by incubating PLGA microspheres in simulated body fluid. The apatite that coated the PLGA microsphere surfaces was similar to apatite in natural bone, as demonstrated by scanning electron microscopy, X-ray diffraction spectra, energy-dispersive spectroscopy, and Fourier transformed-infrared spectroscopy analyses. Rat osteoblasts were mixed with apatite-coated PLGA microspheres and injected immediately into subcutaneous sites of athymic mice. Osteoblast transplantation with plain PLGA microspheres served as a control. Histological analysis of the implants at 6 weeks with hematoxylin and eosin staining, Masson's trichrome staining, and von Kossa staining revealed much better regeneration of bone in the apatite-coated PLGA microsphere group than the plain PLGA microsphere group. The new bone formation area and the calcium content of the implants were significantly higher in the apatite-coated PLGA microsphere group than in the plain PLGA microsphere group. This study demonstrates the feasibility of using apatite-coated PLGA microspheres as an injectable scaffold for in vivo bone tissue engineering. This scaffold may be useful for bone regeneration through minimally invasive surgical procedures in orthopedic applications. (C) 2007 Wiley Periodicals, Inc.