Transmembrane Protein 166 Expression in Esophageal Squamous Cell Carcinoma in Xinjiang, China
Transmembrane Protein 166 Expression in Esophageal Squamous Cell Carcinoma in Xinjiang, China
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DOI：
10.7314/apjcp.2012.13.8.3713
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发表时间：
2012-01-01
影响因子：
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通讯作者：
Ding, Jian-Bing
中科院分区：
文献类型：
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作者：
Sun, Wei;Ma, Xiu-Min;Ding, Jian-Bing
目的：跨膜蛋白166（TMEM 166）在食管鳞状细胞癌（ESCC）和远端正常食管组织中的表达进行了检查，以评估在肿瘤生物学中的任何作用。方法：采用RT-PCR方法检测36例食管鳞癌组织（36例肿瘤组织，36例远端正常食管组织）中TMEM 166 mRNA的表达。TMEM 166蛋白表达通过免疫组织化学分析来自相同病例的石蜡包埋组织样品。结果如下：半定量分析显示食管鳞癌中TMEM 166 mRNA的表达显著低于远端正常食管组织（0.759 +/- 0.713 vs. 2.622 +/- 1.690，P=0.014）。TMEM 166蛋白表达也显著降低（69.4% vs. 94.4%，P
Objective: Transmembrane protein 166 (TMEM166) expression in esophageal squamous cell carcinoma (ESCC) and remote normal esophageal tissues was examined to assess any role in tumour biology. Methods: TMEM166 mRNA expression in 36 cases with ESCC (36 tumour samples, 36 remote normal esophageal tissue samples) was detected by RT-PCR. TMEM166 protein expression was analysed in paraffin-embedded tissue samples from the same cases by immunohistochemistry. Results: Semi-quantitative analysis showed TMEM166 mRNA expression in ESCCs to be significantly lower than in remote normal esophageal tissues (0.759 +/- 0.713 vs. 2.622 +/- 1.690, P=0.014). TMEM166 protein expression was also significantly reduced (69.4% vs. 94.4%, P