Primary structures of two types of alpha-subunit of rat brain Na+,K+,-ATPase deduced from cDNA sequences.
Primary structures of two types of alpha-subunit of rat brain Na+,K+,-ATPase deduced from cDNA sequences.
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从 cDNA 序列推导出大鼠脑 Na,K,-ATP 酶两种类型 α 亚基的一级结构。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
M. Nakao
中科院分区：
文献类型：
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作者：
Y. Hara;O. Urayama;K. Kawakami;H. Nojima;H. Nagamune;T. Kojima;T. Ohta;K. Nagano;M. Nakao
以人Na ~+，K ~+-ATP酶α亚基的cDNA片段为探针，筛选了大鼠脑cDNA文库。获得并分析了两个不同的cDNA克隆。其中一个是编码哇巴因弱敏感的大鼠肾型Na ~+，K ~+-ATP酶α亚基的cDNA。推导的氨基酸序列由1，018个氨基酸组成。大鼠肾型Na ~+，K ~+-ATP酶α亚基与人、羊、猪的Na ~+，K ~+-ATP酶α亚基氨基酸序列同源性为97%，与电鳐的Na ~+，K ~+-ATP酶α亚基氨基酸序列同源性为87%。基于在弱哇巴因敏感的大鼠肾型酶和哇巴因敏感的人、羊、猪或电鳐酶之间的α-亚基的胞外结构域处的氨基酸序列的比较，提出只有两个显著的氨基酸替换是大鼠肾型α-亚基所特有的。获得的另一个cDNA克隆与原大鼠肾型Na ~+，K ~+-ATP酶α亚基cDNA的核苷酸序列有72%的同源性，推导的氨基酸序列与大鼠肾型Na ~+，K ~+-ATP酶α亚基的氨基酸序列有85%的同源性。
A rat brain cDNA library was screened by using as a probe a fragment of cDNA encoding the alpha-subunit of human Na+,K+-ATPase. Two different cDNA clones were obtained and analyzed. One of them was concluded to be a cDNA encoding the alpha-subunit of the weakly ouabain-sensitive rat kidney-type Na+,K+-ATPase. The deduced amino acid sequence consists of 1,018 amino acids. The alpha-subunit of the rat kidney-type Na+,K+-ATPase shows 97% homology in amino acid sequence with the alpha-subunit of human, sheep, or pig enzyme and 87% with that of Torpedo. Based on a comparison of the amino acid sequence at the extracellular domain of the alpha-subunit between weakly ouabain-sensitive rat kidney-type enzyme and the ouabain-sensitive human, sheep, pig, or Torpedo enzyme, it was proposed that only two significant amino acid replacements are unique to the rat kidney-type alpha-subunit. Another cDNA clone obtained showed 72% homology in nucleotide sequence with the former cDNA coding the alpha-subunit of the rat kidney-type Na+,K+-ATPase and the deduced amino acid sequence exhibited 85% homology with that of the alpha-subunit of rat kidney-type Na+,K+-ATPase.