252Cf‐Plasma Desorption Mass Spectrometry
252Cf‐Plasma Desorption Mass Spectrometry
复制标题
252Cf-等离子体解吸质谱法
DOI：
10.1002/mas.1280040403
复制
发表时间：
1985
影响因子：
6.6
通讯作者：
R. Macfarlane
中科院分区：
文献类型：
--
作者：
B. Sundqvist;R. Macfarlane
各种形式的粒子诱导解吸方法用于非挥发性和热不稳定生物分子的质谱学研究，对质谱学领域产生了强烈的影响。这些方法只使用了大约十年。在20世纪70年代初，人们认为即使是精氨酸这样的小生物分子也很难获得质谱图。现在可以研究分子量高达25,000的蛋白质。第一个被介绍的方法是252Cf-等离子体解吸质谱(PDMS)，它利用来自252Cf源的高能快离子(即裂变碎片)轰击固体样品。1974年，麦克法兰和他的同事首次报道了这种方法。本综述旨在描述该技术的发展，
The introduction of all forms of particle-induced desorption methods for mass spectroscopic investigations of nonvolatile and thermally unstable biomolecules has had a strong impact on the field of mass spectrometry. These methods have only been used for about ten years. In the early 1970s it was considered difficult to get mass spectra even for small biomolecules like arginine. Proteins of molecular weights up to 25,000 can now be studied. The first of these methods that was introduced was 252Cf-Plasma Desorption Mass Spectrometry (PDMS) exploiting high-energy fast ions (ie, fission fragments) from a 252Cf-source to bombard solid samples. The method was reported for the first time in 1974 by Macfarlane and co-workers. The present review is intended to give a description of the development of this technique,