RECENT RUBELLA-VIRUS INFECTION INDICATED BY A LOW AVIDITY OF SPECIFIC IGG
RECENT RUBELLA-VIRUS INFECTION INDICATED BY A LOW AVIDITY OF SPECIFIC IGG
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DOI：
10.1007/bf00917569
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发表时间：
1988-05-01
影响因子：
9.1
通讯作者：
SEPPALA, I
中科院分区：
文献类型：
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作者：
HEDMAN, K;SEPPALA, I
急性期血清中风疹特异性IgG在放射溶血试验中产生特征性改变区，称为“软溶血”。在这里，软溶血被证明是从急性期血清中分离纯化的IgG1亚类的产物。相比之下，从既往风疹免疫血清中分离的IgG1产生普通溶血，说明IgG亚类组成不参与软性溶血的机制。建立了一种新型的固相免疫分析法，用于检测病毒特异性IgG的亲和力。急性期IgG（伴有软溶血）在酶免疫测定（EIA）中被氢键破坏剂从风疹抗原中分离出来，而之前免疫的IgG（表现为普通溶血）大部分保持结合。这些数据表明，软溶血的机制是风疹特异性IgG的贪婪。采用169名受试者的血清对新的定量贪婪度EIA进行了测试。原发性风疹后数周或数月采集的血清可显示近期感染。
Rubella-specific IgG in acute-phase sera produces a characteristically altered zone termed "soft hemolysis" in the radial hemolysis test. Here, the soft hemolysis was shown to be a product of the purified IgG1 subclass isolated from acute-phase sera. In contrast, ordinary hemolysis was produced by IgG1 isolated from sera of previous rubella immunity, indicating that the subclass composition of IgG was not involved in the mechanism of soft hemolysis. A novel type of solid-phase immunoassay was developed for the avidity of virus-specific IgG. Acute-phase IgG (with soft hemolysis) was dissociated from rubella antigen in an enzyme immunoassay (EIA) test by hydrogen-bond disrupting agents under conditions where IgG of previous immunity (showing ordinary hemolysis) remained mostly bound. These data suggest that the mechanism of soft hemolysis is the avidity of rubella-specific IgG. The new quantitative avidity EIA was tested with sera taken from 169 subjects. Recent infection could be shown from sera taken weeks or months after primary rubella.