Phospholipase C activation and diacylglycerol kinase inactivation lead to an increase in diacylglycerol content in spontaneously hypertensive rat.
Phospholipase C activation and diacylglycerol kinase inactivation lead to an increase in diacylglycerol content in spontaneously hypertensive rat.
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磷脂酶 C 激活和二酰甘油激酶失活导致自发性高血压大鼠中二酰甘油含量增加。
DOI：
10.1016/0006-291x(87)90808-4
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发表时间：
1987
影响因子：
3.1
通讯作者：
T. Takenawa
中科院分区：
文献类型：
--
作者：
H. Kato;T. Takenawa
本文测定了自发性高血压大鼠（SHR）和正常血压Wistar京都大鼠（WKY）红细胞血影中磷脂酰肌醇（PI）、磷脂酰肌醇4-磷酸（PIP）和甘油二酯（DG）三种激酶及磷脂酶C的活性。SHR的PI激酶活性显著高于WKY，而PIP激酶活性在SHR和WKY之间无显著差异。在SHR中，水解PIP 2的磷脂酶C的活性也增加。而SHR的DG激酶活性则相反。这些结果表明，有一种趋势，积累DG在SHR。事实上，SHR红细胞中DG含量比WKY增加1.7倍。由于DG是蛋白激酶C的生理激活剂，这种DG的积累可能导致SHR中蛋白激酶C的持续激活。
Activities of three kinases, phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and diacylglycerol (DG) kinases, and phospholipase C were measured in erythrocyte ghosts from spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). PI kinase activity was significantly higher in SHR than WKY but there was no significant difference in PIP kinase activity between SHR and WKY. The activity of phospholipase C, which hydrolyzes PIP2, was also increased in SHR. However, DG kinase activity was, on the contrary, decreased in SHR. These results suggest that there is a tendency to accumulate DG in SHR. Indeed, DG content in erythrocytes of SHR increased 1.7-fold compared to that of WKY. Such DG accumulation may cause the sustained activation of protein kinase C in SHR, since DG is a physiological activator for protein kinase C.