A novel thiolated human-like collage zinc complex as a promising zinc supplement: physicochemical characteristics and biocompatibility.
A novel thiolated human-like collage zinc complex as a promising zinc supplement: physicochemical characteristics and biocompatibility.
复制标题
DOI：
10.1016/j.msec.2014.08.010
复制
发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
Chenhui Zhu;Xiaoxuan Ma;Yonghui Wang;Y. Mi;Daidi Fan;Jianjun Deng;Wenjiao Xue
中科院分区：
文献类型：
--
作者：
Chenhui Zhu;Xiaoxuan Ma;Yonghui Wang;Y. Mi;Daidi Fan;Jianjun Deng;Wenjiao Xue
为了提高锌与类人胶原（HLC）的结合能力和金属配合物的稳定性，在pH 8.0条件下，用巯基琥珀酸酐（S-AMSA）对HLC进行巯基化。当pH为8.0，锌离子浓度为15 mM时，每摩尔巯基化HLC-Zn（SHLC-Zn）配合物含有24.3 mol锌离子。采用紫外-可见光谱、圆二色谱、电泳分析、差示扫描量热法（DSC）和细胞活力测定等方法研究了巯基化HLC-Zn（SHLC-Zn）配合物的理化性质和生物相容性。结果表明，SHLC-Zn复合物（1）锌离子含量高于天然HLC，但锌离子与SHLC发生相互作用后仍保持HLC的二级结构;（2）表观分子量较天然HLC增加;（3）热稳定性较天然HLC高;（4）对BHK细胞无毒。本研究表明SHLC-锌复合物是一种潜在的营养品，也是一种潜在的补锌剂。
To improve zinc binding ability to human-like collagen (HLC) and stability of metal complex, HLC was thiolated by mercaptosuccinylation reaction with S-acetylmercaptosuccinic anhydride (S-AMSA) at pH 8.0. One mole of thiolated HLC–Zn (SHLC–Zn) complex possessed 24.3 mol zinc ions when pH was 8.0 and zinc concentration was 15 mM. The physicochemical properties and biocompatibility of thiolated HLC–Zn (SHLC–Zn) complex were investigated by UV–vis, CD, electrophoresis analysis, differential scanning calorimetry (DSC) and cell viability assay, respectively. The results showed that SHLC–Zn complex(1) exhibited higher zinc ions than that of native HLC and still maintained the secondary structure of HLC though interaction occurred between SHLC and zinc ions, (2) increased the apparent molecular weight when compared with native HLC, (3) exhibited greater thermal stability than native HLC, and (4) presented toxicity free for BHK cells. This study suggests that the SHLC–Zn complex is a potential nutrition as well as zinc supplement in the medical application.