The β-barrel assembly machinery in motion.
The β-barrel assembly machinery in motion.
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DOI：
10.1038/nrmicro.2016.191
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发表时间：
2017-04
期刊：
影响因子：
--
通讯作者：
Buchanan SK
中科院分区：
文献类型：
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作者：
Noinaj N;Gumbart JC;Buchanan SK
在革兰氏阴性细菌中，β-桶外膜蛋白的生物发生是由 β-桶组装机器 (BAM) 复合体介导的。在过去的十年中，结构和功能研究共同促进了我们对 BAM 复杂结构和功能的理解；然而，确切的机制仍然难以捉摸。在这篇进展文章中，我们讨论了最近的结构研究，这些研究表明辅助蛋白可能在功能过程中调节核心成分 BamA 内必要的前所未有的构象变化。我们还回顾了从结构数据、诱变研究、分子动力学模拟中获得的机制见解，并探索了细菌中 BAM 介导的 OMP 生物发生的两种新兴模型。在这篇进展文章中，Buchanan 和同事讨论了最近的结构研究，这些研究增进了我们对完全组装的 β 桶组装机械 (BAM) 复合体的结构以及各个组件之间的相互作用的理解。他们还回顾了已经获得的机制见解，并探索了细菌中 BAM 介导的 OMP 生物发生的两种新兴模型。
In Gram-negative bacteria, the biogenesis of β-barrel outer membrane proteins is mediated by the β-barrel assembly machinery (BAM) complex. Over the past decade, structural and functional studies have collectively contributed to advancing our understanding of the BAM complex structure and function; however the exact mechanism remains elusive. In this Progress article, we discuss recent structural studies that have revealed that the accessory proteins may regulate essential unprecedented conformational changes within the core component BamA during function. We also review the mechanistic insights that have been gained from structural data, mutagenesis studies, molecular dynamics simulations, and explore two emerging models for BAM-mediated OMP biogenesis in bacteria. In this Progress article, Buchanan and colleagues discuss recent structural studies that have advanced our understanding of the structure of the fully assembled β-barrel assembly machinery (BAM) complex and the interactions between the individual components. They also review the mechanistic insights that have been gained and explore two emerging models for BAM-mediated OMP biogenesis in bacteria.