Biochemical, molecular characterization and growth promoting effects of phosphate solubilizing Pseudomonas sp isolated from weeds grown in salt range of Pakistan
Biochemical, molecular characterization and growth promoting effects of phosphate solubilizing Pseudomonas sp isolated from weeds grown in salt range of Pakistan
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DOI：
10.1007/s11104-010-0372-8
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发表时间：
2010-09-01
期刊：
影响因子：
4.9
通讯作者：
Bano, Asghari
中科院分区：
文献类型：
--
作者：
Naz, Irum;Bano, Asghari
从莴苣（Lactuca dissecta D.）根中分离到3株解磷细菌，即门多萨假单胞菌（Pseudomonasmendocina）Khsr 2、施氏假单胞菌（Pseudomonasstutzeri）Khsr 3和恶臭假单胞菌（Pseudomonasputida）Khsr 4。唐，白茄f和苦苣菜（Sonchus arvensis L.）分别生长在Khewra盐区（EC：2.3dS m（-1）; pH 8.6）。根据形态学和生化特征对分离菌株进行了初步鉴定，并通过16 S-rRNA基因部分测序进行了验证。利用随机扩增多态性DNA指纹技术分析了各菌株间的遗传多样性，并计算了相似性矩阵。这些假单胞菌均能解磷，在培养基中产生吲哚乙酸、赤霉素、反式玉米素核苷和脱落酸等植物激素，并能有效地促进玉米（高级种质系Islamabad Gold）幼苗在正常和NaCl（20 dS m（-1））胁迫下的根冠长度、干重和脯氨酸含量。施氏假单胞菌Khsr 3是一种潜在的盐碱地生物菌剂。
Three species of phosphate solubilizing bacteria viz, Pseudomonas mendocina Khsr2, Pseudomonas stutzeri Khsr3 and Pseudomonas putida Khsr4 were isolated from roots of weeds Lactuca dissecta D. Don, Solanum surattense Burm. f and Sonchus arvensis L. respectively growing in Khewra salt range (EC: 2.3 dS m(-1); pH 8.6). Preliminary identification of bacterial isolate was made on the basis of morphological and biochemical characters and confirmed by partial 16S-rRNA gene sequencing. The genetic diversity among the isolates was evaluated by Randomly Amplified Polymorphic DNA finger printing and similarity matrix was measured. All the Pseudomonas sp. were capable of solubilizing phosphate, produced phytohormones: Indole-3-acetic acid, Gibberellic acid, Trans-zeatin riboside and Abscisic acid in culture media and were found to be efficient in stimulating root/shoot length and dry weight and proline contents of Zea mays L (advance germplasm line: Islamabad Gold) seedlings grown under normal and NaCl (20 dS m(-1)) stress. The strain Pseudomonas stutzeri Khsr3 appears to be a potential candidate as bio-inoculant for saline fields.