In Vitro Development of Human Killer-Immunoglobulin Receptor-Positive NK Cells
In Vitro Development of Human Killer-Immunoglobulin Receptor-Positive NK Cells
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DOI：
10.1007/978-1-60761-362-6_2
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发表时间：
2010-01-01
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影响因子：
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通讯作者：
Miller, Jeffrey S.
中科院分区：
文献类型：
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作者：
Cichocki, Frank;Miller, Jeffrey S.
本章概述的体外培养系统允许描绘CD34(+)造血祖细胞向成熟的KIR+人NK细胞发育过程中发生的事件。该系统还可用于通过逆转录病毒转导和长期培养来研究基因过表达或基因敲除对NK细胞分化过程的影响。在过去的16年里，我们实验室已经研究出了人体NK细胞体外发育所必需的可溶性因子和接触依赖条件。
The in vitro culture system outlined in this chapter allows for the delineation of events that Occur during the development of CD34(+) hematopoietic precursor cells into mature KIR+ human NK cells. This system can also be utilized to study the effects of gene overexpression or knockdown on the process of NK cell differentiation through retroviral transduction and long-term culture. The necessary soluble factors and contact-dependent conditions for in vitro human NK cell development have been worked out in our laboratory over the past 16 years.