Anti-Inflammatory Effects of Gomisin N, Gomisin J, and Schisandrin C Isolated from the Fruit of Schisandra chinensis
Anti-Inflammatory Effects of Gomisin N, Gomisin J, and Schisandrin C Isolated from the Fruit of Schisandra chinensis
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DOI：
10.1271/bbb.90597
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发表时间：
2010-02-01
影响因子：
1.6
通讯作者：
Lee, Jae Kwon
中科院分区：
文献类型：
--
作者：
Oh, Su-Yeon;Kim, Young Hoon;Lee, Jae Kwon
五味子是治疗肝病的著名中药。在本研究中，我们研究了五味子的九种主要化合物是否可以用于抑制脂多糖（LPS）诱导的小鼠巨噬细胞（Raw 264.7细胞）的炎症反应。在九种木酚素中，其中三种（戈米辛 J、戈米辛 N 和五味子素 C）被发现可以减少 LPS 刺激的 Raw 264.7 细胞中一氧化氮 (NO) 的产生。这三种木酚素在 Raw 264.7 细胞中表现出较低的细胞毒性作用。用戈米辛 I、戈米辛 N 或五味子 C 预处理 Raw 264.7 细胞可减少 mRNA 的表达和促炎细胞因子的分泌。这些抑制作用被发现是由 p38 丝裂原激活蛋白激酶 (MAPK)、细胞外信号调节激酶 1 和 2 (ERK 1/2) 以及 c-Jun N 末端激酶 (JNK) 磷酸化的阻断引起的。
Schiandra chinensis is a well-known Chinese traditional medicine for the treatment of hepatic disease. In this study, we investigated whether the nine major compounds of Schiandra chinensis could be applied to suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Among the nine lignans, three, gomisin J, gomisin N, and schisandrin C, were found to reduce nitric oxide (NO) production from LPS-stimulated Raw 264.7 cells. These three lignans showed low cytotoxic effects in Raw 264.7 cells. Pre-treatment of Raw 264.7 cells with gomisin I, gomisin N, or schisandrin C reduced the expression of mRNA and the secretion of pro-inflammatory cytokines. These inhibitory effects were found to he caused by blockage of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), and c-Jun N-terminal kinase (JNK) phosphorylation.