Tailoring the Mechanical Stiffness of DNA Nanostructures Using Engineered Defects
Tailoring the Mechanical Stiffness of DNA Nanostructures Using Engineered Defects
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DOI：
10.1021/acsnano.9b03770
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发表时间：
2019-07-01
期刊：
影响因子：
17.1
通讯作者：
Kim, Do-Nyun
中科院分区：
文献类型：
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作者：
Lee, Chanseok;Kim, Kyung Soo;Kim, Do-Nyun
由于支架DNA折纸能够构建具有预定形状的多种DNA纳米结构，因此精确调节其机械刚度仍然具有挑战性。我们展示了一种模块化设计方法，以广泛和精确地控制支架DNA折纸纳米结构的机械灵活性，同时保持其整体结构的完整性和几何特征。单独设计的短单链DNA（ssDNA）缺口缺陷可以降低具有不同横截面形状的DNA折纸结构的弯曲刚度高达70%。我们进一步开发了一个计算分析平台，在设计过程中快速预测缺陷工程化DNA纳米结构的弯曲刚度，以提供一种有效的方法来设计具有所需机械刚度的各种DNA构建体，以实现目标功能。
As scaffolded DNA origami enables the construction of diverse DNA nanostructures with predefined shapes, precise modulation of their mechanical stiffness remains challenging. We demonstrate a modular design method to widely and precisely control the mechanical flexibility of scaffolded DNA origami nanostructures while maintaining their overall structural integrity and geometric characteristics. Individually engineered defects that are short single-stranded DNA (ssDNA) gaps could reduce up to 70% of the bending stiffness of DNA origami constructs with different cross-sectional shapes. We further developed a computational analysis platform predicting the bending stiffness of a defect-engineered DNA nanostructure quickly during the design process, to offer an efficient way of designing various DNA constructs with required mechanical stiffness in a desired shape for a targeted function.