Rhamnolipids are conserved biosurfactants molecules: implications for their biotechnological potential
Rhamnolipids are conserved biosurfactants molecules: implications for their biotechnological potential
复制标题
DOI：
10.1007/s00253-013-4876-z
复制
发表时间：
2013-08-01
影响因子：
5
通讯作者：
Banat, Ibrahim M.
中科院分区：
文献类型：
--
作者：
Perfumo, Amedea;Rudden, Michelle;Banat, Ibrahim M.
从广泛不同的环境来源的铜绿假单胞菌的一系列分离株的合成鼠李糖脂生物表面活性剂的能力进行了检查。没有显着差异的数量或组成的鼠李糖脂同系物可以通过操纵生长条件。鼠李糖脂基因序列表明菌株变异水平较低，大多数多态性确实导致氨基酸序列变化，但没有明显的表型效应。rhlB和rhlC鼠李糖基转移酶基因的表达在生长过程中表现出固定的顺序表达模式，并且通过不同的生产菌株或生长培养基不能诱导显著的上调。结果表明，鼠李糖脂是高度保守的分子，其基因表达有相当严格的控制。这使得几乎没有机会操纵并大大增加来自铜绿假单胞菌菌株的鼠李糖脂的产量以用于生物技术应用。
A range of isolates of Pseudomonas aeruginosa from widely different environmental sources were examined for their ability to synthesise rhamnolipid biosurfactants. No significant differences in the quantity or composition of the rhamnolipid congeners could be produced by manipulating the growth conditions. Sequences for the rhamnolipid genes indicated low levels of strain variation, and the majority of polymorphisms did lead to amino acid sequence changes that had no evident phenotypic effect. Expression of the rhlB and rhlC rhamnosyltransferase genes showed a fixed sequential expression pattern during growth, and no significant up-regulation could be induced by varying producer strains or growth media. The results indicated that rhamnolipids are highly conserved molecules and that their gene expression has a rather stringent control. This leaves little opportunity to manipulate and greatly increase the yield of rhamnolipids from strains of P. aeruginosa for biotechnological applications.