Neurotrophic property of geniposide for inducing the neuronal differentiation of PC12 cells
Neurotrophic property of geniposide for inducing the neuronal differentiation of PC12 cells
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京尼平苷诱导 PC12 细胞神经元分化的神经营养特性
DOI：
10.1016/j.ijdevneu.2006.08.009
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发表时间：
2006-11-01
影响因子：
1.8
通讯作者：
Zhang, Heng
中科院分区：
文献类型：
--
作者：
Liu, Jianhui;Zheng, Xuxu;Zhang, Heng
新的数据表明，胰岛素营养激素胰高血糖素样肽-1（GLP-1）及其激动剂extendin-4具有神经营养功能，可诱导PC12细胞的神经元分化，并防止氧化应激引起的神经元损伤。在此，我们利用GLP-1受体激动剂的高通量筛选模型，筛选并鉴定出京尼平苷是一种新型的GLP-1受体激动剂。此外，京尼平苷还可诱导 PC12 细胞的神经元分化，从而导致神经突生长。我们还观察到生长相关蛋白 43 的表达增加。 U0126 是一种选择性 MEK 抑制剂，可防止京尼平苷诱导的 PC12 细胞中神经突的生长和丝裂原激活激酶蛋白的磷酸化。这些结果表明京尼平苷激活GLP-1受体诱导PC12细胞神经元分化涉及MAPK信号级联反应。 (c) 2006 年由 Elsevier Ltd 代表 ISDN 出版。
The emerging data show that the insulinotrophic hormone glucagon-like peptide-1(GLP-1) and its agonist extendin-4 have neurotrophic function to inducing neuronal differentiation of PC12 cells and prevent neurons damage challenged by oxidative stress. Here, with the model of high throughput screen for GLP-1 receptor agonists, we screen and identify that geniposide is a novel agonist for GLP-1 receptor. Furthermore, geniposide induces the neuronal differentiation of PC12 cells with resulting neurites outgrowth; we also observe an increase in expression of growth-associated protein-43. U0126, a selective MEK inhibitor, prevents neurites out growth and phosphorylation of mitogen-activated kinase proteins in PC12 cells induced by geniposide. All these results show that activation of GLP-1 receptor by geniposide to induce the neuronal differentiation of PC12 cells involves in MAPK signaling cascade. (c) 2006 Published by Elsevier Ltd on behalf of ISDN.