Collagen-Binding Human Epidermal Growth Factor Promotes Cellularization of Collagen Scaffolds
Collagen-Binding Human Epidermal Growth Factor Promotes Cellularization of Collagen Scaffolds
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DOI：
10.1089/ten.tea.2008.0648
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发表时间：
2009-11-01
影响因子：
4.1
通讯作者：
Dai, Jianwu
中科院分区：
文献类型：
--
作者：
Yang, Yifan;Zhao, Yannan;Dai, Jianwu
此前的研究表明，表皮生长因子（EGF）可以有效促进组织再生。本研究的目的是将胶原蛋白结合域（CBD）与天然EGF（NAT-EGF）结合形成融合蛋白，旨在使其能够特异性结合胶原蛋白支架。尽管有细胞外液，这种方法仍可以限制 EGF 的扩散，并限制 EGF 向周围组织的释放。因此，保留的 CBD-EGF 将在组织再生过程中提供持续的功能。在我们的研究中，体外生物活性测定表明CBD-EGF和NAT-EGF在刺激人成纤维细胞增殖方面没有显着差异。在胶原蛋白结合测定中，与 NAT-EGF 相比，CBD-EGF 表现出对胶原蛋白的特异性结合能力。最后，体内实验表明，与 NAT-EGF 相比，加载 CBD-EGF 的胶原膜均具有较高的促进支架细胞化的能力。上述所有结果表明，负载 CBD-EGF 的胶原蛋白是一种具有潜在临床应用的功能性生物材料。
Previous studies have demonstrated that epidermal growth factor (EGF) can promote tissue regeneration effectively. The aim of the present study was to combine a collagen-binding domain (CBD) with native EGF (NAT-EGF) to form a fusion protein, aiming at that it could bind to collagen scaffolds specifically. This approach could restrict the diffusion of EGF despite of extracellular fluids and limit the release of EGF to surrounding tissues. Thus, the retained CBD-EGF would render a continual function during the tissue regeneration. In our study, in vitro bioactivity assay showed that there was no significant difference between CBD-EGF and NAT-EGF to stimulate human fibroblasts to proliferate. In the collagen-binding assay, CBD-EGF exhibited a specific binding ability to collagen compared to NAT-EGF. Finally, in vivo experiment demonstrated that CBD-EGF loaded collagen membranes uniformly possess a high capability to promote cellularization of scaffolds as compared with that of NAT-EGF. All results aforementioned suggested that collagen loaded with CBD-EGF is a functional biomaterial with potential clinical applications.