IMMUNOCHEMICAL STUDIES ON THE ROLE OF THE GOLGI-COMPLEX IN PROTEIN-BODY FORMATION IN RICE SEEDS
IMMUNOCHEMICAL STUDIES ON THE ROLE OF THE GOLGI-COMPLEX IN PROTEIN-BODY FORMATION IN RICE SEEDS
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DOI：
10.1007/bf00392095
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发表时间：
1986-01-01
期刊：
影响因子：
4.3
通讯作者：
OKITA, TW
中科院分区：
文献类型：
--
作者：
KRISHNAN, HB;FRANCESCHI, VR;OKITA, TW
以纯化的谷蛋白和醇溶谷蛋白的抗体为探针，研究了这些蛋白沉积到水稻蛋白体中的细胞途径。胚乳三种形态不同的蛋白体，大的球形，小的球形，和不规则形状，观察到，与现有的报告。免疫细胞化学研究表明，存在谷蛋白的不规则形状的蛋白体，而醇溶谷蛋白被发现在大，小的球形蛋白体。无论是大的和小的球形蛋白体，可区分的电子密度和金标记模式，似乎是由直接沉积的新形成的蛋白质到内腔的粗面内质网（ER）。相反，谷蛋白体是通过高尔基体形成的。小的电子透明囊泡经常出现在高尔基体的一侧。电子致密囊泡，其内容物被谷蛋白抗体-金颗粒标记，通常在高尔基体的远侧观察到，并且在胚乳细胞中融合形成不规则形状的蛋白体。这些观察结果表明，水稻谷蛋白从其合成位点，ER，到沉积位点，蛋白体的运输，是由高尔基体介导。
Antibodies raised against purified glutelins and prolamines were employed as probes to study the cellular routes by which these proteins are deposited into protein bodies of rice (Oryza sativa L.) endosperm. Three morphologically distinct protein bodies, large spherical, small spherical, and irregularly-shaped, were observed, in agreement with existing reports. Immunocytochemical studies showed the presence of glutelins in the irregularly-shaped protein bodies while the prolamines were found in both the large and small spherical protein bodies. Both the large and small spherical protein bodies, distinguishable by electron density and gold-labeling patterns, appear to be formed by direct deposition of the newly formed proteins into the lumen of the rough endoplasmic reticulum (ER). In contrast, glutelin protein bodies are formed via the Golgi apparatus. Small electron-lucent vesicles are often found at one side of the Golgi. Electron-dense vesicles, whose contents are labeled by glutelin antibody-gold particles, are commonly observed at the distal side of the Golgi apparatus and fuse to form the irregularly shaped protein bodies in endosperm cells. These observations indicate that the transport of rice glutelins from their site of synthesis, the ER, to the site of deposition, the protein bodies, is mediated by the Golgi apparatus.