Measuring biological age in mice using differential mass spectrometry
Measuring biological age in mice using differential mass spectrometry
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DOI：
10.18632/aging.101810
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发表时间：
2019-02-15
期刊：
影响因子：
5.2
通讯作者：
Yates, Nathan A.
中科院分区：
文献类型：
--
作者：
Bell-Temin, Harris;Yousefzadeh, Matthew J.;Yates, Nathan A.
老龄化是一个不明确的过程，增加了发病率和死亡率的风险。衰老也是异质的，这意味着生物学和实足年龄可能不同。在这里，我们使用无偏差分质谱法定量小鼠肝脏中的数千种蛋白质，并选择那些随着小鼠年龄增长表达持续变化的蛋白质。来自近交系C57 BL/6小鼠的一组14种蛋白质用于使该参考群体中的实足年龄和生物学年龄相等，可以与其他小鼠进行比较。这个“生物年龄计算器”确定了两种f1杂交小鼠在生物学上比近交小鼠年轻，而早衰小鼠在生物学上比近交小鼠年长。在一项独立的验证实验中，计算器识别出用雷帕霉素治疗的小鼠，已知可以延长小鼠的寿命，比用安慰剂饮食喂养的小鼠年轻18%。这表明，使用蛋白质组学方法测量哺乳动物生物年龄的细微变化是可能的。
Aging is an ill-defined process that increases the risk of morbidity and mortality. Aging is also heterogeneous meaning that biological and chronological age can differ. Here, we used unbiased differential mass spectrometry to quantify thousands of proteins in mouse liver and select those that that consistently change in expression as mice age. A panel of 14 proteins from inbred C57BL/6 mice was used to equate chronological and biological age in this reference population, against which other mice could be compared. This "biological age calculator" identified two strains of f1 hybrid mice as biologically younger than inbred mice and progeroid mice as being biologically older. In an independent validation experiment, the calculator identified mice treated with rapamycin, known to extend lifespan of mice, as 18% younger than mice fed a placebo diet. This demonstrates that it is possible to measure subtle changes in biologic age in mammals using a proteomics approach.