Epidermal growth factor stimulates transcription of the c-jun proto-oncogene in rat fibroblasts
Epidermal growth factor stimulates transcription of the c-jun proto-oncogene in rat fibroblasts
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DOI：
10.1038/334538a0
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发表时间：
1988-08
期刊：
影响因子：
64.8
通讯作者：
B. Quantin;R. Breathnach
中科院分区：
文献类型：
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作者：
B. Quantin;R. Breathnach
一些生长因子诱导的基因，如c-fosgene，在没有干预蛋白质合成的情况下被迅速和短暂地激活。其他基因，如大鼠转运素基因2，激活速度较慢，但更持久，它们的激活需要事先蛋白质合成。人们很容易推测，某些快速激活的基因编码转录因子，这些转录因子直接与转录素基因等基因的启动子区域相互作用，从而触发它们的表达。不幸的是，对支持这一假说的大多数主要反应基因知之甚少。原癌基因c-jun编码转录因子AP-1或密切相关的蛋白3，4。我们发现，表皮生长因子刺激成纤维细胞中c-jun基因的转录是其主要反应。这支持了这样一种观点，即编码转录因子的基因的表达增加是信号转导机制的重要元素，确保了细胞对生长因子的长期转录反应。
Some growth factor-induced genes, such as the c-fosgene, are activated rapidly and transiently without intervening protein synthesis1. Others, like the rat transin gene2, are activated more slowly but more durably and their activation requires prior protein synthesis. It is tempting to speculate that certain rapidly-activated genes code for transcription factors which interact directly with promoter regions of genes like the transin gene to trigger their expression. Unfortunately, little is known about the majority of primary response genes to support this hypothesis. The proto-oncogene c-juncodes for the transcription factor AP-1 or a closely related protein3,4. We show that epidermal growth factor stimulates transcription of the c-jungene in fibroblasts as a primary response. This supports the notion that increased expression of genes encoding transcription factors is an important element of the signal transduction mechanism, assuring the long-term transcriptional response of cells to growth factors.