Changes in surface architecture during murine erythroleukemia cell differentiation as detected by lectin binding and agglutination.
Changes in surface architecture during murine erythroleukemia cell differentiation as detected by lectin binding and agglutination.
复制标题
通过凝集素结合和凝集检测小鼠红白血病细胞分化过程中表面结构的变化。
DOI：
10.1016/0005-2736(81)90014-6
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Sartorelli,AC
中科院分区：
文献类型：
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作者：
Sartorelli,AC
细胞表面的变化发生在小鼠红白血病细胞（克隆745）分化，检测凝集和凝集素结合。红白血病细胞的凝集是由麦胚凝集素产生的;而刀豆球蛋白A、蓖麻毒素、大豆凝集素和岩藻糖结合蛋白则是无效的或效果差得多的。用红系分化诱导剂二甲基亚砜（DMSO）处理白血病细胞，导致培养物中含血红蛋白的细胞逐渐积累，麦胚凝集素的凝集率降低，该凝集率在暴露于极性溶剂后24 h开始，在48 h达到最低点，此后基本保持不变。未处理的对照红白血病细胞的放射性麦胚凝集素的结合随着培养时间的增加，在48小时达到最大值，此后逐渐下降。尽管DMSO处理的细胞中3 H标记的麦胚凝集素结合也增加，但在24-96 h，结合水平显著低于对照细胞中观察到的水平。用不同浓度的DMSO处理红白血病细胞导致麦胚凝集素受体位点数量减少。其他分化诱导物（即，二甲基甲酰胺、双（乙酰基）二氨基戊烷）也抑制麦胚凝集素诱导的红白血病细胞凝集的速率，而与此相反，诱导剂四甲基脲则不抑制。这些研究表明，膜的变化发生在分化过程中，并表明可能有一个以上的机制参与成熟的启动，最终导致红细胞发育的共同途径。
Cell surface alterations occurred during murine erythroleukemia cell (clone 745) differentiation that were detected by both agglutination and lectin binding. Agglutination of erythroleukemia cells was produced by wheat germ agglutinin; whereas, concanavalin A, Ricin, soybean agglutinin and fucose-binding protein were either ineffective or much less efficacious. Treatment of leukemia cells with the inducer of erythroid differentiation dimethylsulfoxide (DMSO) caused a progressive accumulation of hemoglobin-containing cells in culture and a decrease in the rate of agglutination by wheat germ agglutinin, which began at 24 h after exposure to the polar solvent, reached a nadir at 48 h, and remained essentially constant thereafter. The binding of radioactive wheat germ agglutinin by untreated control erythroleukemia cells increased with time in culture, reaching a maximum value at 48 h, and decreased progressively thereafter. Although an increase in3H-labeled wheat germ agglutinin binding also occurred in DMSO-treated cells, the level bound was significantly lower than that observed in control cells at 24–96 h. The treatment of erythroleukemia cells with various concentrations of DMSO resulted in a decrease in the number of wheat germ agglutinin receptor sites. Other inducers of differentiation (i.e., dimethylformamide, bis(acetyl)diaminopentane) also inhibited the rate of wheat germ agglutinin-induced agglutination of erythroleukemia cells while, in contrast, the inducer tetramethylurea did not. These studies indicate that membrane changes occur during differentiation and suggest that there may be more than one mechanism involved in the initiation of maturation which ultimately leads to the common pathway of erythroid development.