In Vivo Imaging of Sentinel Nodes Using Fluorescent Silica Nanoparticles in Living Mice
In Vivo Imaging of Sentinel Nodes Using Fluorescent Silica Nanoparticles in Living Mice
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DOI：
10.1007/s11307-009-0262-8
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发表时间：
2010-04-01
影响因子：
3.1
通讯作者：
Kang, Keon Wook
中科院分区：
文献类型：
--
作者：
Jeon, Yong Hyun;Kim, Young-Hwa;Kang, Keon Wook
我们研究了功能化二氧化硅纳米颗粒用于前哨淋巴结检测的可行性，并开发了一种功能化的RITC-SiO2纳米颗粒，其中含有荧光染料C28 H31 N2 O3 Cl（罗丹明B异硫氰酸酯），随后合成Ga-68-NOTA-RITC-SiO2纳米颗粒。在活体小鼠的右腋窝淋巴结（ALN）和注射部位均显示荧光信号。在生物分布研究中，在这些位置也观察到荧光信号。此外，在显微镜下在冷冻ALN切片中检测到荧光。用Ga-68-NOTA-RITC-SiO2纳米颗粒处理的足垫附近的腋窝和臂淋巴结的每克组织注射的剂量百分比分别为308.3 +/- 3.4和41.5 +/- 6.1。Ga-68在其他器官中的放射性很小。我们的数据提供了强有力的证据，表明功能化的二氧化硅纳米颗粒在生物医学成像中作为有机淋巴示踪剂具有很好的潜力，例如术前和术中手术指导。
We examine the feasibility of fluorescent imaging system for sentinel lymph node detection by using functionalized silica nanoparticles.We developed a functionalized RITC-SiO2 nanoparticles containing fluorescent dye, C28H31N2O3Cl (rhodamine B isothiocyanate) inside, and subsequently synthesized Ga-68-NOTA-RITC-SiO2 nanoparticles.At 5 min after RITC-doped silica nanoparticles injection, fluorescent signals were shown in both right axillary lymph node (ALN) and injection site of living mice. Fluorescent signals were also observed at these locations in a biodistribution study. In addition, fluorescence was detected in frozen ALN sections microscopically. The percentages of doses injected per gram of tissue of axillary and brachial lymph nodes near footpad treated with Ga-68-NOTA-RITC-SiO2 nanoparticles were 308.3 +/- 3.4 and 41.5 +/- 6.1, respectively. Little Ga-68 radioactivity was found in other organs.Our data provide strong evidence that functionalized silica nanoparticles has a promising potential as organic lymphatic tracer in biomedical imaging such as pre- and intraoperative surgical guidance.