CRR23/NdhL is a subunit of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis.
CRR23/NdhL is a subunit of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis.
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DOI：
10.1093/pcp/pcn058
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发表时间：
2008-05
影响因子：
4.9
通讯作者：
H. Shimizu;Lianwei Peng;F. Myouga;R. Motohashi;K. Shinozaki;T. Shikanai
中科院分区：
文献类型：
--
作者：
H. Shimizu;Lianwei Peng;F. Myouga;R. Motohashi;K. Shinozaki;T. Shikanai
在高等植物中，叶绿体NAD(P)H脱氢酶(NDH)复合体在PSI循环和氯呼吸电子传递中起作用。到目前为止，已经鉴定出11个叶绿体编码的亚基和3个核编码的亚基，但整个亚基的组成，特别是假定的电子供体结合模块，尚不清楚。根据光化光照后Chl荧光没有瞬时增强的特点，我们分离到了缺乏NDH活性的拟南芥crr23(氯呼吸减少)突变体。虽然CRR23与蓝藻NDH-1的NdhL亚基相似，但它有三个跨膜结构域，而不是蓝藻NdhL的两个跨膜结构域。与蓝藻NdhL不同，CRR23对于稳定NDH复合体是必不可少的，而NDH复合体又是CRR23积累所必需的。此外，CRR23和叶绿体NDH的一个亚基NdhH在蓝色天然凝胶中共定位。这些结果表明，CRR23是拟南芥中蓝藻ndhL的同源基因，尽管其结构和功能具有多样性。
The chloroplast NAD(P)H dehydrogenase (NDH) complex functions in PSI cyclic and chlororespiratory electron transport in higher plants. Eleven plastid-encoded and three nuclear-encoded subunits have been identified so far, but the entire subunit composition, especially of the putative electron donor-binding module, is unclear. We isolated Arabidopsis thaliana crr23 (chlororespiratory reduction) mutants lacking NDH activity according to the absence of a transient increase in Chl fluorescence after actinic light illumination. Although CRR23 shows similarity to the NdhL subunit of cyanobacterial NDH-1, it has three transmembrane domains rather than the two in cyanobacterial NdhL. Unlike cyanobacterial NdhL, CRR23 is essential for stabilizing the NDH complex, which in turn is required for the accumulation of CRR23. Furthermore, CRR23 and NdhH, a subunit of chloroplast NDH, co-localized in blue-native gel. All the results indicate that CRR23 is an ortholog of cyanobacterial ndhL in Arabidopsis, despite its diversity of structure and function.