Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Glucagon bound to perdeuterated dodecylphosphocholine micelles.
Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Glucagon bound to perdeuterated dodecylphosphocholine micelles.
复制标题
蛋白质 1H 核磁共振谱中的顺序共振分配。
DOI：
10.1016/0022-2836(82)90010-9
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发表时间：
1982
影响因子：
5.6
通讯作者：
K. Wüthrich
中科院分区：
文献类型：
--
作者：
G. Wider;K. H. Lee;K. Wüthrich
本文用二维氢核磁共振技术对胰高血糖素与全氘代十二烷基磷酸胆碱胶束结合的氢核磁共振谱进行了归属。除了丝氨酸2的N-末端氨基和酰胺质子外，获得了所有骨架和Cβ质子的顺序共振归属。除了Gln 20和Gln 24的γ-亚甲基质子外，非不稳定氨基酸侧链质子的归属是完整的。这些分配为确定脂质结合胰高血糖素的三维结构提供了基础。
The assignment of the1H nuclear magnetic resonance spectrum of glucagon bound to perdeuterated dodecylphosphocholine micelles with the use of two-dimensional1H nuclear magnetic resonance techniques at 360 MHz is described. Sequential resonance assignments were obtained for all backbone and Cβprotons except the N-terminal amino group and the amide proton of Ser2. The assignments of the non-labile amino acid side-chain protons are complete except for the γ-methylene protons of Gln20 and Gln24. These assignments provide a basis for the determination of the three-dimensional structure of lipid-bound glucagon.