Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method
Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method
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用简单的消减法分离水稻低温诱导蛋白基因
DOI：
10.1093/oxfordjournals.pcp.a078207
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发表时间：
1991
影响因子：
4.9
通讯作者：
T. Kusano
中科院分区：
文献类型：
--
作者：
K. Aguan;K. Sugawara;N. Suzuki;T. Kusano
我们已经分离的cDNA，对应于三个不同的低温诱导的mRNA从水稻细胞使用一种有效的，简单的减法。相应的基因被命名为lip（编码低温诱导蛋白的基因）-5、-9和-19。这些基因的表达受到脱落酸的轻微刺激（lip 5和lip 19）或不受影响（lip 9），而不像脱落酸或高渗透胁迫容易诱导的therab 16 A-D基因的表达。时程分析显示，在温度下降后3 h，lip 5基因被诱导，而lip 9和lip 19基因在温度下降后6 h被诱导。
We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designatedlip(gene encoding low-temperature-induced protein) −5,−9 and −19. The expression of these genes was slightly stimulatedlip5andlip19)or unaffected(lip9)by abscisic acid, unlike the expression of therabl6A-Dgenes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while thelip5gene was induced 3 h after temperature down-shift, and thelip9andlipl9genes were induced 6 h after such a shift.