Sucrose and starch catabolism in the anther of Lilium during its development:: a comparative study among the anther wall, locular fluid and microspore/pollen fractions
Sucrose and starch catabolism in the anther of Lilium during its development:: a comparative study among the anther wall, locular fluid and microspore/pollen fractions
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DOI：
10.1007/s00425-006-0443-5
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发表时间：
2007-05-01
期刊：
影响因子：
4.3
通讯作者：
Clement, Christophe
中科院分区：
文献类型：
--
作者：
Castro, Antonio J.;Clement, Christophe
为了更好地了解百合花药中蔗糖和淀粉酶的各种途径，我们对百合花药中蔗糖和淀粉酶的合成进行了研究。“Enchantment”）、转化酶（EC 3.2.1.26）和淀粉酶（EC 3.2.1.1，EC 3.2.1.2）活性分别在不同组分（花药壁、腔液和小孢子/花粉）中测量，并与花药发育期间的糖含量相关。我们的研究结果表明，分析的馏分之间的显着差异，表明蔗糖和淀粉催化剂的调节可以遵循不同的途径，在每个馏分。葡萄糖和果糖含量从花药壁到花药液和从花药液到小孢子/花粉逐渐降低。因此，发育中的花粉可以充当到达花药的碳水化合物的库。从这个意义上说，细胞壁结合的转化酶似乎发挥了重要作用，可溶性糖分配在花药的不同部分。蔗糖浓度被发现是实质上高于在其他部分的腔室流体，表明一个可能的存储位置。另一方面，花药壁组织具有缓冲作用，将多余的营养物质储存在淀粉粒中，从而调节整个花药中可溶性糖的利用率。这些结果证明了所建立的实验模型的优越性，以及该模型在百合花药糖代谢研究中的实用性。
In order to better understand the various pathways of sucrose and starch catabolism in the anther of lily (Lilium hybrida var. "Enchantment"), invertase (EC 3.2.1.26) and amylase (EC 3.2.1.1, EC 3.2.1.2) activities were measured separately in different fractions (anther wall, locular fluid and microspore/pollen) and correlated with the sugar content during anther development. Our findings showed significant differences among the fractions analyzed, suggesting that the regulation of sucrose and starch catabolism could follow distinct pathways in each fraction. Glucose and fructose amounts progressively decreased from anther wall to fluid and from fluid to microspore/pollen. Thus, the developing pollen could act as a sink for the carbohydrates that reach the anther. In this sense, cell wall-bound invertases seem to play a major role in soluble sugar partitioning in the different fractions of the anther. Sucrose concentration was found to be substantially higher in the locular fluid than in the other fractions, indicating a probable site for storage. On the other hand, the anther wall tissues could have a buffering function, storing nutrient surplus in starch grains and thus regulating the availability of soluble sugars in the whole anther. All these results proved the advantages of the experimental model proposed here, as well as its usefulness to investigate sugar metabolism in Lilium anthers.