Mucins and Ocular Signs in Symptomatic and Asymptomatic Contact Lens Wear
Mucins and Ocular Signs in Symptomatic and Asymptomatic Contact Lens Wear
复制标题
DOI：
10.1097/opx.0b013e318188896b
复制
发表时间：
2008-10-01
影响因子：
1.4
通讯作者：
Murphy, Paul J.
中科院分区：
文献类型：
--
作者：
Berry, Monica;Pult, Heiko;Murphy, Paul J.
目的。睑擦拭器上皮病 (LWE) 和睑平行结膜皱襞 (LIPCOF) 与隐形眼镜佩戴者的干眼症状有关。这两种临床症状都被认为与眨眼时的机械力有关。由于粘液层是眼表组织的保护层，因此本研究调查了比较有症状和无症状的软隐形眼镜佩戴者是否可以检测到粘蛋白的任何变化。方法。使用隐形眼镜干眼问卷评估舒适度。对 50 名（19 名男性，31 名女性；平均年龄，32.1 +/- 11.4 岁）有经验的镜片配戴者的右眼进行了角膜染色、LWE 和 LIPCOF 评估。使用压在颞结膜上的 Schirmer 条带和从收获的隐形眼镜上对泪膜进行取样。在 1% 琼脂糖或 4 至 12% NuPAGE 凝胶上电泳后，通过斑点印迹和蛋白质印迹评估粘蛋白。非参数分析用于研究组间差异以及客观测试、粘蛋白和症状之间的相关性。结果。通过问卷调查，31 名受试者被分类为无症状，19 名受试者被分类为有症状。有症状组的 LWE 和 LIPCOF 显着增加 (p < 0.035)。 MUC5AC 反应性在症状中显着降低 (p = 0.050)。 MUC4 与颞叶 LIPCOF 和 LWe 相关（r = -0.47 和 -0.46；p < 0.01）。 MUC16 和 MUC5AC 与角膜染色相关（0.36 < r < 0.53；p < 0.04）。结论。有症状的隐形眼镜佩戴者表现出明显更高的 LWE 和 LIPCOF，并且 MUC5AC 反应性降低。 LWE与LIPCOF显着相关；这可能反映了它们共同的摩擦起源。摩擦力增加可能是由于粘蛋白不足或眼表面粘蛋白成分改变所致。在这项研究中，我们发现粘蛋白产生的减少与 LWE 和 LIPCOF 的严重程度相关。 （光学可见科学 2008 年；85：930-938）
Purpose. Lid wiper epitheliopathy (LWE) and lid parallel conjunctival folds (LIPCOF) are related to dry eye symptoms in contact lens wearers. Both clinical signs are assumed to he related to mechanical forces during blinking. As the mucus layer is a protector of the Ocular Surface tissue, this study investigates whether any alterations of mucins are detectable comparing symptomatic and asymptomatic soft contact lens wearers.Methods. Comfort was evaluated using the Contact Lens Dry Eye Questionnaire. Corneal staining, LWE, and LIPCOF were assessed in the right eyes of 50 (19 men, 31 women; mean age, 32.1 +/- 11.4 years) experienced lens wearers. The tear film was sampled using Schirmer strips pressed onto the temporal Conjunctiva and from harvested contact lenses. Mucins were assessed in dot-blots and Western blots after electrophoresis on 1% agarose or 4 to 12%, NuPAGE Gels. Non-parametric analyses were Used to study differences between groups and correlations between objective tests, mucins, and symptoms.Results. Thirty-one subjects were classified asymptomatic and 19 symptomatic by the questionnaire. LWE and LIPCOF were significantly increased in the symptomatic group (p < 0.035). MUC5AC reactivity was significantly decreased in symptomatics (p = 0.050). MUC4 was correlated to temporal LIPCOF and LWe, (r = -0.47 and -0.46; p < 0.01). MUC16 and MUC5AC correlated with corneal staining (0.36 < r < 0.53; p < 0.04).Conclusions. Symptomatic contact lens wearers exhibit significantly more LWE and LIPCOF,, and decreased MUC5AC reactivity. LWE and LIPCOF are significantly correlated; this may reflect their common frictional origin. Increased friction might follow from insufficient mucins, or an altered composition of the resident mucins at the ocular surface. In this study, we show that decreased mucin production is associated with the severity of LWE and LIPCOF. (Optom Vis Sci 2008;85:930-938)