A Novel Pectin-Like Glycopeptide Isolated from the Fruit of Fructus Mori Impedes Aggregation and Production of Aβ42
A Novel Pectin-Like Glycopeptide Isolated from the Fruit of Fructus Mori Impedes Aggregation and Production of Aβ42
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从桑果中分离出的新型果胶样糖肽可阻碍 Aβ42 的聚集和产生
DOI：
10.1021/acs.jafc.2c03823
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Kan Ding
中科院分区：
文献类型：
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作者：
Saijuan Li;Can Jin;Pengcheng Gao;Wanqi Zhou;Rongrong Xiao;Zhenqiang Zhang;Wenfeng Liao;Kan Ding
桑葚的果实是食品和药物，已被证明具有显着的神经保护作用。然而，其有效成分尚不清楚。我们推测，水果提取物中的糖肽具有类似的活性。为了解决这一假设，我们从果实中分离出一种新的果胶样糖肽（FMP-6-S4），分子量为11.23 kDa。它含有约20%由17个氨基酸组成的肽和80%由l -鼠李糖（L-Rha）、d-半乳糖（D-Gal）、d-半乳糖醛酸（D-GalA）、l -阿拉伯糖（L-Ara）和d-葡萄糖（D-Glc）组成的聚糖，摩尔比为7.25:4.62:77.66:5.62:4.85。多糖部分的主链由1,4-链α-D-GalpA和1,2 -链α-L-Rhap组成，分支由取代部分半乳糖醛酸C-3位的己醛酸（HexA）组成，鼠李糖C-4位的半乳糖、葡萄糖和阿拉伯糖组成。体外实验表明，FMP-6-S4可能通过调节淀粉样前体蛋白（APP）加工，抑制a - β42 （β-淀粉样肽42）聚集，减少a - β42的产生。
The fruit of Fructus Mori is food and medicine, which has been demonstrated to have a significant neuroprotective effect. However, the effective constituent remains unknown. We speculate that the glycopeptide in the extract of the fruit has similar activity. To address this hypothesis, we isolated a novel pectin-like glycopeptide (FMP-6-S4) with a molecular weight of 11.23 kDa from the fruit. It contains about 20% of peptide comprising 17 amino acids and 80% glycan consisting of L-rhamnose (L-Rha), D-galactose (D-Gal), D-galacturonic acid (D-GalA), L-arabinose (L-Ara) and d-glucose (D-Glc) in molar ratios of 7.25:4.62:77.66:5.62:4.85. The backbone of the glycan part consisted of 1,4-linked α-D-GalpA and 1, 2-linked α-L-Rhap, while the branches were composed of hexenuronic acid (HexA) substituted at the C-3 position of partial galacturonic acid, and traces of galactose, glucose, and arabinose were substituted at the C-4 position of rhamnose. The in vitro experiments revealed that FMP-6-S4 might inhibit Aβ42 (β-amyloid peptides 42) aggregation and decrease Aβ42 production by modulating APP (amyloid precursor protein) processing.