Pathogenic Characteristics of and Variation in Vibrio parahaemolyticus Isolated from Acute Diarrhoeal Patients in Southeastern China from 2013 to 2017
Pathogenic Characteristics of and Variation in Vibrio parahaemolyticus Isolated from Acute Diarrhoeal Patients in Southeastern China from 2013 to 2017
复制标题
2013-2017年东南地区急性腹泻患者副溶血性弧菌致病特征及变化
DOI：
10.2147/idr.s234186
复制
发表时间：
2020-01-01
影响因子：
3.9
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Chen, Xiao;Zhu, Qiaoyun;Chen, Yu
目的：副溶血弧菌是沿海地区主要的腹泻病原菌。本研究分析了2013年至2017年中国东南部不同地区7家医院急性腹泻患者分离的副溶血性弧菌的病原学特征和变异情况。方法：收集急性腹泻患者的粪便标本。采用常规微生物检测程序结合MALDI Biotyper微生物鉴定系统对副溶血性弧菌进行鉴定。进行血清凝集试验、毒力相关基因PCR检测和药敏试验Kirby-Bauer法。结果：2013年至2017年中国东南部地区16504份急性腹泻患者粪便标本中共分离出副溶血弧菌1220株，年分离率波动在6.1%~8.7%之间。夏秋季节共分离出96.7%的副溶血弧菌，主要集中在18-44岁人群中。鉴定出59种血清型，0抗原凝集率为98.5%。 2014年至2016年，优势血清型为03：K6，而2013年和2017年，优势血清型为04：KUT。 03:K6、04:KUT、04:K8、03:KUT、010:K60、01:KUT和01:K36血清型从2013年至2017年每年都会出现。04:K6和OUT:K6分别在2014年和2015年后开始出现。共有49.5%的菌株属于大流行组，该组由26个血清型组成。大多数分离株对除氨苄西林以外的常用抗生素敏感。结论：副溶血性弧菌在中国东南部地区仍处于高水平。尽管大流行中03:K6血清型占主导地位，但新的血清型不断出现，特别是04:KUT血清型，其流行率在某些年份超过了03:K6。为了防止这种病原体的爆发或传播，需要进行长期监测。
Objective: Vibrio parahaemolyticus is a major diarrhoea-inducing pathogen in coastal areas. In this study, we analysed the pathogenic characteristics of and variation in V. parahaemolyticus isolated from acute diarrhoeal patients in seven hospitals in different areas of southeastern China from 2013 to 2017.Methods: The fecal specimens of patients with acute diarrhoea were collected. The routine microbiological test procedure combining with MALDI Biotyper microbial identification system was carried out to identify the V. parahaemolyticus. Serum agglutination tests, PCR for the detection of virulence-related genes and the Kirby-Bauer method to test for antimicrobial sensitivity were performed.Results: From 2013 to 2017 in southeastern China, a total of 1220 V. parahaemolyticus strains were isolated from 16,504 stool specimens collected from acute diarrhoeal patients, and the annual isolation rate fluctuated between 6.1% and 8.7%. In total, 96.7% of the V. parahaemolyticus isolates were isolated in summer and autumn, mainly in people aged 18-44. Fifty-nine serotypes were identified, and the agglutination rate of the 0 antigen was 98.5%. From 2014 to 2016, the dominant serotype was 03:K6, while in 2013 and 2017, it was 04:KUT. The serotypes of 03:K6, 04:KUT, 04:K8, 03:KUT, 010:K60, 01:KUT and 01:K36 appeared every year from 2013 to 2017. 04:K6 and OUT:K6 began to appear after 2014 and 2015, respectively. A total of 49.5% of the strains belonged to the pandemic group, which consisted of 26 serotypes. Most isolates were sensitive to common antibiotics, excluding ampicillin.Conclusion: V. parahaemolyticus is still present at a high level in southeastern China. Although the pandemic 03:K6 serotype is predominant, new serotypes continue to emerge, especially the 04:KUT serotype, which exceeded 03:K6 in prevalence in some years. Long-term surveillance is necessary to prevent the outbreak or transmission of this pathogen.