Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation.
Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation.
复制标题
控制细胞质聚腺苷酸化的序列元件的进化保守性。
DOI：
10.1073/pnas.93.17.9027
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发表时间：
1996
影响因子：
11.1
通讯作者：
Wickens,M
中科院分区：
文献类型：
--
作者：
Verrotti,AC;Thompson,SR;Wreden,C;Strickland,S;Wickens,M
细胞质多聚腺苷酸化是一种进化保守的机制，在发育早期参与一组母体信使RNA(MRNAs)的翻译激活。在这份报告中，我们通过物种间注射证明了非洲爪哇和小鼠在减数分裂成熟过程中使用相同的调控序列来控制细胞质PolyA的添加。同样，非洲爪哇和果蝇胚胎在受精后发育的早期利用功能保守的信号来调节多聚腺苷酸化。这些实验证明，控制细胞质聚腺苷酸化的序列元件，从而一种形式的翻译激活，在不同物种之间发挥作用。我们推断，自从脊椎动物和节肢动物的分化以来，必需的调控序列元件，以及可能与它们相互作用的反式作用元件，一直是保守的。
Cytoplasmic polyadenylylation is an evolutionarily conserved mechanism involved in the translational activation of a set of maternal messenger RNAs (mRNAs) during early development. In this report, we show by interspecies injections that Xenopus and mouse use the same regulatory sequences to control cytoplasmic poly(A) addition during meiotic maturation. Similarly, Xenopus and Drosophila embryos exploit functionally conserved signals to regulate polyadenylylation during early post-fertilization development. These experiments demonstrate that the sequence elements that govern cytoplasmic polyadenylylation, and hence one form of translational activation, function across species. We infer that the requisite regulatory sequence elements, and likely the trans-acting components with which they interact, have been conserved since the divergence of vertebrates and arthropods.