Identification of potential target genes for the neuron-restrictive silencer factor
Identification of potential target genes for the neuron-restrictive silencer factor
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DOI：
10.1073/pnas.93.18.9881
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发表时间：
1996-09-03
影响因子：
11.1
通讯作者：
Anderson, DJ
中科院分区：
文献类型：
--
作者：
Schoenherr, CJ;Paquette, AJ;Anderson, DJ
神经元限制性沉默因子（NRSF）通过与21bp的神经元限制性沉默因子（NRSE）结合，抑制非神经元细胞中几种神经元基因的转录。我们使用复合NRSE进行数据库搜索，以确定其他候选NRSF靶基因。另外22个基因，其中17个主要在神经元中表达，被发现含有nrse样序列。许多这些假定的nrse在体外结合NRSF，并在体内抑制转录。大多数已确定的神经元基因都与神经元的基本结构或功能特性有关。然而，两个神经元转录因子基因含有nrse，这表明NRSF可能直接或间接地抑制神经元分化。在一些非神经元基因中也发现了功能性NRSF，这意味着NRSF可能发挥比最初预期更广泛的作用。
The neuron-restrictive silencer factor (NRSF) represses transcription of several neuronal genes in nonneuronal cells by binding to a 21-bp element called the neuron-restrictive silencer element (NRSE). We have performed data base searches with a composite NRSE to identify additional candidate NRSF target genes. Twenty-two more genes, 17 of which are expressed mainly in neurons, were found to contain NRSE-like sequences. Many of these putative NRSEs bound NRSF in vitro and repressed transcription in vivo. Most of the neuronal genes identified contribute to the basic structural or functional properties of neurons. However, two neuronal transcription factor genes contain NRSEs, suggesting that NRSF may repress neuronal differentiation both directly and indirectly. Functional NRSEs were also found in several nonneuronal genes, implying that NRSF may play a broader role than originally anticipated.