Expression of HYAL1 and Survivin RNA as Diagnostic Molecular Markers for Bladder Cancer
Expression of HYAL1 and Survivin RNA as Diagnostic Molecular Markers for Bladder Cancer
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DOI：
10.1016/j.juro.2009.10.024
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发表时间：
2010-02-01
影响因子：
6.6
通讯作者：
El-Ahmady, Omar
中科院分区：
文献类型：
--
作者：
Eissa, Sanaa;Swellam, Menha;El-Ahmady, Omar
目的：有助于膀胱癌早期检测的尿液肿瘤标志物有望在敏感性，特异性和便利性方面比传统的侵入性诊断测试有显着提高。我们评估了透明质酸酶（HYAL 1）和生存素的早期膀胱癌detect.Materials和方法：本研究包括166例诊断为膀胱癌，112例良性膀胱病变和100名健康志愿者作为对照组的诊断效率。所有患者均进行血吸虫病血清学抗体检测、尿细胞学检查、尿上皮细胞透明质酸酶（HYAL 1）和存活素RNA定性和半定量逆转录聚合酶链反应（RT-PCR）检测。采用半定量方法计算的平均秩次在恶性组中比其他组增加。HYAL 1和生存素RNA的最佳截止值均为0.25。使用这些截断值，HYAL 1和生存素RNA的敏感性分别为91%和75%，具有绝对的特异性。HYAL 1 RNA检测到所有0期和I期膀胱癌患者（p < 0.037）。尿细胞学检查的敏感性提高时，结合透明质酸酶或生存素RNA的半定量RT-PCR.Conclusions：尿HYAL 1和生存素RNA的检测是一个有前途的非侵入性检测膀胱癌的早期检测。HYAL 1 RNA比尿脱落细胞学更敏感、特异。半定量逆转录-聚合酶链反应因其高度的敏感性和特异性而备受青睐。
Purpose: Urinary tumor markers that help in the early detection of bladder cancer promise a significant improvement in sensitivity, specificity and convenience over conventional, invasive diagnostic tests. We assessed the diagnostic efficacy of hyaluronidase (HYAL1) and survivin for early bladder cancer detection.Materials and Methods: The study included 166 patients diagnosed with bladder carcinoma, 112 with benign bladder lesions and 100 healthy volunteers who served as controls. All underwent serological assessment of schistosomiasis antibody, urine cytology, and hyaluronidase (HYAL1) and survivin RNA estimation by qualitative and semiquantitative reverse transcriptase-polymerase chain reaction in urothelial cells from voided urine.Results: Positivity rates of HYAL1 RNA and survivin RNA on qualitative reverse transcriptase-polymerase chain reaction were significantly different among the 3 groups. Mean rank using semiquantitative method was increased in the malignant vs the other groups. The best cutoff for HYAL1 and survivin RNA was 0.25 each. Using these cutoffs HYAL1 and survivin RNA sensitivity was 91% and 75%, respectively, with absolute specificity. HYAL1 RNA detected all patients with stages 0 and I bladder cancer (p < 0.037). Urine cytology sensitivity improved when combined with hyaluronidase or survivin RNA on semiquantitative reverse transcriptase-polymerase chain reaction.Conclusions: The detection of urinary HYAL1 and survivin RNA is a promising noninvasive test for bladder cancer early detection. HYAL1 RNA was more sensitive and specific than urine cytology. Semiquantitative reverse transcriptase-polymerase chain reaction is favored for its high sensitivity and specificity.